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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera)
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Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera)

机译：与蜜蜂菌落崩溃障碍有关的转录本丰度变化（Apis mellifera）

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摘要

Colony collapse disorder (CCD) is a mysterious disappearance of honey bees that has beset beekeepers in the United States since late 2006. Pathogens and other environmental stresses, including pesticides, have been linked to CCD, but a causal relationship has not yet been demonstrated. Because the gut acts as a primary interface between the honey bee and its environment as a site of entry for pathogens and toxins, we used whole-genome microar-rays to compare gene expression between guts of bees from CCD colonies originating on both the east and west coasts of the United States and guts of bees from healthy colonies sampled before the emergence of CCD. Considerable variation in gene expression was associated with the geographical origin of bees, but a consensus list of 65 transcripts was identified as potential markers for CCD status. Overall, elevated expression of pesticide response genes was not observed. Genes involved in immune response showed no clear trend in expression pattern despite the increased prevalence of viruses and other pathogens in CCD colonies. Microarray analysis revealed unusual ribosomal RNA fragments that were conspicuously more abundant in the guts of CCD bees. The presence of these fragments may be a possible consequence of picorna-like viral infection, including deformed wing virus and Israeli acute paralysis virus, and may be related to arrested translation. Ribosomal fragment abundance and presence of multiple viruses may prove to be useful diagnostic markers for colonies afflicted with CCD.
机译：自2006年底以来，菌落崩溃障碍（CCD）是蜜蜂在美国养蜂人的神秘消失。病原体和其他环境压力（包括农药）与CCD相关，但尚未发现因果关系。由于肠道是蜜蜂与其环境之间的主要界面，是环境中病原体和毒素进入的场所，因此我们使用全基因组微射线来比较源自东部和东部的CCD殖民地的蜜蜂肠道之间的基因表达。在美国西海岸以及在CCD出现之前从健康菌落中提取的蜂胆。基因表达的显着变化与蜜蜂的地理起源有关，但共有65个转录本的共有列表被鉴定为CCD状态的潜在标记。总体而言，未观察到农药反应基因的表达升高。尽管在CCD菌落中病毒和其他病原体的流行率增加，但参与免疫反应的基因却没有明显的表达方式变化。基因芯片分析显示，不寻常的核糖体RNA片段在CCD蜜蜂的肠道中明显更丰富。这些片段的存在可能是像picorna一样的病毒感染的可能结果，包括变形的机翼病毒和以色列的急性瘫痪病毒，并且可能与翻译被阻止有关。核糖体片段的丰度和多种病毒的存在可能被证明是受CCD侵袭的菌落的有用诊断标记。

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    Reed M. Johnson; Jay D. Evans; Gene E. Robinson; May R. Berenbaum;

  • 作者单位

    Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL 61801 Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE 68583;

    Bee Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705;

    Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL 61801;

    Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL 61801;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    microarray; picorna-like virus; ribosomal RNA;

    机译：微阵列皮科尔纳样病毒;核糖体RNA;

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