...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Coherent X-ray diffraction from collagenous soft tissues
【24h】

Coherent X-ray diffraction from collagenous soft tissues

机译：胶原软组织的相干X射线衍射

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Coherent X-ray diffraction has been applied in the imaging of inorganic materials with great success. However, its application to biological specimens has been limited to some notable exceptions, due to the induced radiation damage and the extended nature of biological samples, the last limiting the application of most part of the phasing algorithms. X-ray ptychography, still under development, is a good candidate to overcome such difficulties and become a powerful imaging method for biology. We describe herein the feasibility of applying ptychography to the imaging of biological specimens, in particular collagen rich samples. We report here speckles in diffraction patterns from soft animal tissue, obtained with an optimized small angle X-ray setup that exploits the natural coherence of the beam. By phasing these patterns, dark field images of collagen within tendon, skin, bone, or cornea will eventually be obtained with a resolution of 60-70 nm. We present simulations of the contrast mechanism in collagen based on atomic force microscope images of the samples. Simulations confirmed the 'speckled' nature of the obtained diffraction patterns. Once inverted, the patterns will show the disposition and orientation of the fibers within the tissue, by enhancing the phase contrast between protein and no protein regions of the sample. Our work affords the application of the most innovative coherent X-ray diffraction tools to the study of biological specimens, and this approach will have a significant impact in biology and medicine because it overcomes many of the limits of current microscopy techniques.
机译：相干X射线衍射已成功应用于无机材料的成像。但是，由于诱发的辐射损伤和生物样品的扩展性质，其在生物标本中的应用受到一些明显的限制，最后限制了大部分定相算法的应用。仍在开发中的X射线指纹图谱技术是克服此类困难并成为生物学强大的成像方法的良好选择。我们在本文中描述了将typchography应用于生物样本（尤其是富含胶原蛋白的样本）成像的可行性。我们在这里报告了来自软动物组织的衍射图样中的斑点，这些斑点是通过利用光束的自然相干性进行了优化的小角度X射线设置获得的。通过定相这些图案，最终将以60-70 nm的分辨率获得肌腱，皮肤，骨骼或角膜内胶原蛋白的暗场图像。我们目前基于样品的原子力显微镜图像模拟胶原蛋白中的对比机制。模拟证实了所获得的衍射图案的“斑点”性质。一旦反转，通过增强样品中蛋白质与无蛋白质区域之间的相衬，图案将显示组织内纤维的排列和方向。我们的工作提供了最创新的相干X射线衍射工具在生物学标本研究中的应用，并且该方法克服了当前显微镜技术的许多局限性，因此将对生物学和医学产生重大影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号