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In vivo far-red luminescence imaging of a biomarker based on BRET from Cypridina bioluminescence to an organic dye

机译:基于从蓝藻生物发光到有机染料的BRET的生物标记的体内远红外发光成像

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摘要

We aimed to develop a far-red luminescence imaging technology for visualization of disease specific antigens on cell surfaces in a living body. First, we conjugated a far-red fluorescent indocyanine derivative to biotinylated Cypridina luciferase. This conjugate produced a bimodal spectrum that has long-wavelength bioluminescence emission in the far-red region as a result of bioluminescence resonance energy transfer. To generate a far-red luminescent probe with targeting and imaging capabilities of tumors, we then linked this conjugate to an anti-human Dlk-1 monoclonal antibody via the biotin-avidin interaction. This far-red luminescent probe enabled us to obtain high-resolution microscopic images of live, Dlk-1-expressing Huh-7 cells without an external light source, and to monitor the accumulation of this probe in tumor-bearing mice. Thus this far-red luminescent probe is a convenient analytical tool for the evaluations of monoclonal antibody localization in a living body.
机译:我们旨在开发一种远红外发光成像技术,用于可视化活体细胞表面上的疾病特异性抗原。首先,我们将远红荧光吲哚菁衍生物偶联到生物素化的塞浦路斯藻荧光素酶上。由于生物发光共振能量转移,该缀合物产生了双峰光谱,该双峰光谱在远红区域具有长波长生物发光发射。为了产生具有肿瘤靶向和成像功能的远红外发光探针,我们然后通过生物素-亲和素相互作用将该偶联物连接至抗人Dlk-1单克隆抗体。这种远红外发光探针使我们能够在没有外部光源的情况下获得表达Dlk-1的活Huh-7细胞的高分辨率显微图像,并能够监测这种探针在荷瘤小鼠中的积累。因此,这种远红外发光探针是用于评估生物体内单克隆抗体定位的便捷分析工具。

著录项

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  • 作者单位

    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda 563-8577, Japan;

    Graduate School of Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo 060-8638, Japan;

    Graduate School of Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo 060-8638, Japan;

    LivTech, Inc. 907 Nogawa, Miyamae-ku, Kawasaki 216-0001, Japan;

    LivTech, Inc. 907 Nogawa, Miyamae-ku, Kawasaki 216-0001, Japan;

    Graduate School of Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo 060-8638, Japan;

    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda 563-8577, Japan Graduate School of Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo 060-8638, Japan Research Institute for Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology, 2-17-2-1 Tsukisamu-Higashi, Sapporo 062-8517, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cypridina luciferase; far-red luminescent probe; luciferin; tumor;

    机译:塞浦路斯荧光素酶远红色发光探针;萤光素瘤;

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