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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Nanoparticle-based bio-barcode assay redefines 'undetectable' PSA and biochemical recurrence after radical prostatectomy
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Nanoparticle-based bio-barcode assay redefines 'undetectable' PSA and biochemical recurrence after radical prostatectomy

机译：基于纳米粒子的生物条形码检测重新定义了前列腺癌根治术后的“不可检测的” PSA和生化复发

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摘要

We report the development of a previously undescribed gold nano-particle bio-barcode assay probe for the detection of prostate specific antigen (PSA) at 330 fg/mL, automation of the assay, and the results of a clinical pilot study designed to assess the ability of the assay to detect PSA in the serum of 18 men who have undergone radical prostatectomy for prostate cancer. Due to a lack of sensitivity, available PSA immunoassays are often not capable of detecting PSA in the serum of men after radical prostatectomy. This new bio-barcode PSA assay is ≈300 times more sensitive than commercial immunoassays. Significantly, with the barcode assay, every patient in this cohort had a measurable serum PSA level after radical prostatectomy. Patients were separated into categories based on PSA levels as a function of time. One group of patients showed low levels of PSA with no significant increase with time and did not recur. Others showed, at some point postprostatectomy, rising PSA levels. The majority recurred. Therefore, this new ultrasensitive assay points to significant possible outcomes: (1) The ability to tell patients, who have undetectable PSA levels with conventional assays, but detectable and nonrising levels with the barcode assay, that their cancer will not recur. (2) The ability to assign recurrence earlier because of the ability to measure increasing levels of PSA before conventional tools can make such assignments. (3) The ability to use PSA levels that are not detectable with conventional assays to follow the response of patients to adjuvant or salvage therapies.
机译：我们报告了以前未描述的金纳米颗粒生物条形码检测探针的开发情况，该探针可检测330 fg / mL的前列腺特异性抗原（PSA），检测的自动化以及旨在评估肝癌的临床前瞻性研究的结果检测18位接受前列腺癌根治性前列腺切除术的男性血清中PSA的能力。由于缺乏敏感性，可用的PSA免疫测定法通常不能在前列腺癌根治术后检测男性血清中的PSA。这种新的生物条形码PSA分析的灵敏度比商业免疫分析高300倍。值得注意的是，通过条形码分析，该队列中的每个患者在前列腺癌根治术后均具有可测量的血清PSA水平。根据PSA水平随时间将患者分为几类。一组患者表现出较低的PSA水平，但未随时间明显增加，也未复发。其他人则显示前列腺切除术后的某个时刻，PSA水平升高。多数复发。因此，这种新的超灵敏测定法指出了可能的重大结果：（1）能够告诉患者常规检测法检测不到PSA水平，而条形码检测法检测到且无升高水平的患者不会复发。 （2）之所以能够较早地分配复发，是因为能够在常规工具进行此类分配之前测量PSA的升高水平。 （3）能够使用常规测定法无法检测到的PSA水平来跟踪患者对辅助或挽救疗法的反应。

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    C. Shad Thaxton; Robert Elghanian; Audrey D. Thomas; Savka I. Stoeva; Jae-Seung Lee; Norm D. Smith; Anthony J. Schaeffer; Helmut Klocker; Wolfgang Horninger; Georg Bartsch; Chad A. Mirkin;

  • 作者单位

    Department of Urology, Northwestern University Feinberg School of Medicine, 303 East Chicago, Chicago, IL 60611 Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Gaiter Pavillion, 675 North Saint Clair, 21st Floor, Chicago, IL 60611;

    Department of Chemistry, International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208;

    Department of Urology, Northwestern University Feinberg School of Medicine, 303 East Chicago, Chicago, IL 60611;

    Department of Chemistry, International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208;

    Department of Chemistry, International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208;

    Department of Urology, Northwestern University Feinberg School of Medicine, 303 East Chicago, Chicago, IL 60611 Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Gaiter Pavillion, 675 North Saint Clair, 21st Floor, Chicago, IL 60611;

    Department of Urology, Northwestern University Feinberg School of Medicine, 303 East Chicago, Chicago, IL 60611 Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Gaiter Pavillion, 675 North Saint Clair, 21st Floor, Chicago, IL 60611;

    Department of Urology, Innsbruck Medical University, Innsbruck, Tyrol, Austria;

    Department of Urology, Innsbruck Medical University, Innsbruck, Tyrol, Austria;

    Department of Urology, Innsbruck Medical University, Innsbruck, Tyrol, Austria;

    Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Gaiter Pavillion, 675 North Saint Clair, 21st Floor, Chicago, IL 60611 Department of Chemistry, International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    carcinoma of prostate; prostate specific antigen;

    机译：前列腺癌前列腺特异性抗原;

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