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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Interaction of an autotransporter passenger domain with BamA during its translocation across the bacterial outer membrane
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Interaction of an autotransporter passenger domain with BamA during its translocation across the bacterial outer membrane

机译：自转运蛋白客运结构域与BamA在其跨细菌外膜转运过程中的相互作用

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摘要

Autotransporters are a superfamily of virulence factors produced by Gram-negative bacteria consisting of a large N-terminal extracellular domain ("passenger domain") and a C-terminal β barrel domain ("β domain"). The mechanism by which the passenger domain is translocated across the outer membrane (OM) is unknown. Here we show that the insertion of a small linker into the passenger domain of the Escherichia coli O157:H7 autotransporter EspP effectively creates a translocation intermediate by transiently stalling translocation near the site of the insertion. Using a site-specific photocrosslinking approach, we found that residues adjacent to the stall point interact with BamA, a component of a heterooligomeric complex (Bam complex) that catalyzes OM protein assembly, and that residues closer to the EspP N terminus interact with the periplasmic chaperones SurA and Skp. The EspP-BamA interaction was short-lived and could be detected only when passenger domain translocation was stalled. These results support a model in which molecular chaperones prevent misfolding of the passenger domain before its secretion and the Bam complex catalyzes both the integration of the β domain into the OM and the translocation of the passenger domain across the OM in a C- to N-terminal direction.
机译：自转运蛋白是由革兰氏阴性细菌产生的毒力因子的超家族，所述革兰氏阴性细菌由大的N端细胞外结构域（“客体结构域”）和C端β桶状结构域（“β结构域”）组成。乘客域跨外膜（OM）移位的机制尚不清楚。在这里，我们显示了一个小的接头插入大肠杆菌O157：H7自转运蛋白EspP的客运结构域，可通过在插入位点附近暂时停顿易位有效地产生一个易位中间体。使用位点特异性光交联方法，我们发现失速点附近的残基与BamA相互作用，BamA是催化OM蛋白装配的杂聚体（Bam复合物）的组分，而距EspP N末端更近的残基与周质相互作用。伴侣SurA和Skp。 EspP-BamA相互作用是短暂的，只有在乘客域易位停止时才能检测到。这些结果支持了一种模型，其中分子伴侣在分泌之前阻止了乘客结构域的错误折叠，并且Bam络合物既催化β结构域整合到OM中，又催化乘客结构域在C-到N-之间跨OM转运。终端方向。

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