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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Twenty years of combinatorial antibody libraries, but how well do they mimic the immunoglobulin repertoire?
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Twenty years of combinatorial antibody libraries, but how well do they mimic the immunoglobulin repertoire?

机译：组合抗体文库已有20年的历史，但是它们模仿免疫球蛋白库的效果如何？

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摘要

Combinatorial antibody libraries celebrate their 20th anniversary this year. In 1989, the first functional antibodies were isolated from a combinatorial antibody library derived from an immunized mouse (1). It was immediately evident that the technology would also lend itself to the generation of purely human monoclonal antibodies for medical use, which was not possible to achieve with the cell-based approaches for isolation of monoclonal antibodies used at the time. In these antibody libraries, mRNAs from B lymphocytes, coding for millions or even billions of the antibody heavy and light chains, are rescued by PCR technology and expressed as functional antibodies most often on phage that allow isolation of clones with particular characteristics, e.g., binding to a target molecule, cell type, or tissue (2, 3).
机译：组合抗体文库今年庆祝成立20周年。 1989年，从衍生自免疫小鼠的组合抗体库中分离了第一批功能性抗体（1）。立刻显而易见的是，该技术还将有助于产生纯人类用于医学用途的单克隆抗体，而用基于细胞的方法分离当时使用的单克隆抗体是无法实现的。在这些抗体库中，来自B淋巴细胞的mRNA编码成千上万甚至数十亿的抗体重链和轻链，通过PCR技术得以拯救，并最常在噬菌体上表达为功能性抗体，从而可以分离具有特定特征（例如结合）的克隆靶分子，细胞类型或组织（2，3）。

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