Nickel-inducible lysis system in Synechocystis sp. PCC 6803

Xinyao Liu; Roy Curtiss III

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Nickel-inducible lysis system in Synechocystis sp. PCC 6803

机译：尼氏囊藻中的镍诱导裂解系统。 PCC 6803

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摘要

We designed and constructed a controllable inducing lysis system in Synechocystis sp. PCC 6803 to facilitate extracting lipids for biofuel production. Several bacteriophage-derived lysis genes were integrated into the genome and placed downstream of a nickel-inducible signal transduction system. We applied 3 strategies: (ⅰ) directly using the phage lysis cassette, (ⅱ) constitutively expressing endolysin genes while restricting holin genes, and (ⅲ) combining lysis genes from different phages. Significant autolysis was induced in the Synechocystis sp. PCC 6803 cells with this system by the addition of NiSO_4. Our inducible cyanobacterial lysing system eliminates the need for mechanical or chemical cell breakage and could facilitate recovery of biofuel from cyanobacteria.

机译：我们设计和构建了一个可控制的诱导裂解系统，在集胞藻中。 PCC 6803有助于提取脂质以生产生物燃料。几个噬菌体衍生的裂解基因被整合到基因组中，并置于镍诱导信号转导系统的下游。我们应用了3种策略：（ⅰ）直接使用噬菌体裂解盒，（ⅱ）组成性表达内皮素基因，同时限制holin基因，以及（ⅲ）组合来自不同噬菌体的裂解基因。显着的自溶作用在集胞藻中被诱导。 PCC 6803电池通过在该系统中添加NiSO_4。我们的诱导型蓝细菌裂解系统消除了机械或化学细胞破碎的需要，并可以促进从蓝细菌中回收生物燃料。

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2009年第51期|21550-21554|共5页
作者
Xinyao Liu; Roy Curtiss III;
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作者单位

Center for Infectious Diseases and Vaccinology, The Biodesign Institute and School of Life Sciences, Arizona State University, Tempe, AZ 85287-5401;

Center for Infectious Diseases and Vaccinology, The Biodesign Institute and School of Life Sciences, Arizona State University, Tempe, AZ 85287-5401;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
biofuel; phage lysis genes;

机译：生物燃料噬菌体裂解基因;

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1. Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 [J] . Vita Vidmar, Aneja Tahirovi?, Marko Dolinar, Biology Open . 2016,第4期

机译：生物技术上重要的微藻的生物安全性：蓝藻Synechocystis sp。中固有的自杀开关实施。 PCC 6803生物技术上重要的微藻的生物安全性：蓝藻集胞藻属中固有的自杀开关实施。 PCC 6803生物技术上重要的微藻的生物安全性：蓝藻集胞藻属中固有的自杀开关实施。 PCC 6803
2. Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis sp. PCC 6803 [J] . Vita Vidmar, Aneja Tahirovi?, Marko Dolinar, Biology Open . 2016,第4期

机译：生物技术上重要的微藻的生物安全性：蓝藻Synechocystis sp。中固有的自杀开关实施。 PCC 6803生物技术上重要的微藻的生物安全性：蓝藻集胞藻属中固有的自杀开关实施。 PCC 6803生物技术上重要的微藻的生物安全性：蓝藻集胞藻属中固有的自杀开关实施。 PCC 6803
3. A novel cell lysis system induced by phosphate deficiency in the cyanobacterium Synechocystis sp. PCC 6803 [J] . Asada Ryo, Shiraiwa Yoshihiro, Suzuki Iwane Journal of Applied Phycology . 2019,第2期

机译：磷酸盐缺陷鉴定的一种新型细胞裂解系统在综合症SP中磷酸盐缺乏症。 PCC 6803.
4. SYSTEMS AND SYNTHETIC BIOLOGY ADVANCEMENTS TO IMPROVE SYNECHOCYSTIS SP. PCC 6803 STRAIN ENGINEERING IN THE INDUSTRIALLY-RELEVANT CONDITION OF DIURNAL LIGHT-DARK CYCLES [C] . Christie Peebles Conference on biochemical and molecular engineering . 2019

机译：系统和合成生物学的进步，以提高剑突。日轻暗循环工业相关条件下的PCC 6803应变工程
5. In vitro investigations of the hemoglobins from the bacteria Synechocystis sp. PCC 6803, Synechococcus sp. PCC 7002, and Helicobacter hepaticus. [D] . Nothnagel, Henry J. 2010

机译：细菌Synechocystis sp。的血红蛋白的体外研究。 PCC 6803，Synechococcus sp。 PCC 7002和肝幽门螺杆菌。
6. Nickel-inducible lysis system in Synechocystis sp. PCC 6803 [O] . Xinyao Liu, Roy Curtiss III 2009

机译：尼氏囊藻中的镍诱导裂解系统。 PCC 6803
7. Nickel-inducible lysis system in Synechocystis sp. PCC 6803 [O] . Liu, Xinyao, Curtiss, Roy 2009

机译：尼氏囊藻中的镍诱导裂解系统。 PCC 6803

Nickel-inducible lysis system in Synechocystis sp. PCC 6803

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