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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Characterization of an electron conduit between bacteria and the extracellular environment
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Characterization of an electron conduit between bacteria and the extracellular environment

机译：细菌与细胞外环境之间电子导管的表征

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摘要

A number of species of Gram-negative bacteria can use insoluble minerals of Fe(III) and Mn(IV) as extracellular respiratory electron acceptors. In some species of Shewanella, deca-heme electron transfer proteins lie at the extracellular face of the outer membrane (OM), where they can interact with insoluble substrates. To reduce extracellular substrates, these redox proteins must be charged by the inner membrane/periplasmic electron transfer system. Here, we present a spectro-potentiometric characterization of a trans-OM icosa-heme complex, MtrCAB, and demonstrate its capacity to move electrons across a lipid bilayer after incorporation into proteoliposomes. We also show that a stable MtrAB subcomplex can assemble in the absence of MtrC; an MtrBC subcomplex is not assembled in the absence of MtrA; and MtrA is only associated to the membrane in cells when MtrB is present. We propose a model for the modular organization of the MtrCAB complex in which MtrC is an extracellular element that mediates electron transfer to extracellular substrates and MtrB is a trans-OM spanning β-barrel protein that serves as a sheath, within which MtrA and MtrC exchange electrons. We have identified the MtrAB module in a range of bacterial phyla, suggesting that it is widely used in electron exchange with the extracellular environment.
机译：许多革兰氏阴性细菌可以使用不溶性的Fe（III）和Mn（IV）矿物作为细胞外呼吸电子受体。在希瓦氏菌的某些物种中，十血红素电子转移蛋白位于外膜（OM）的细胞外表面，在那里它们可以与不溶性底物相互作用。为了减少细胞外底物，这些氧化还原蛋白必须由内膜/周质电子转移系统带电。在这里，我们介绍了反式-OM二十面体-血红素复合物MtrCAB的分光光度法表征，并证明了其结合到蛋白脂质体中后，电子跨脂质双层移动的能力。我们还表明，在没有MtrC的情况下，稳定的MtrAB亚复合体可以组装。如果没有MtrA，则不会组装MtrBC亚复合体；当存在MtrB时，MtrA仅与细胞膜相关。我们为MtrCAB复合物的模块化组织提出了一个模型，其中MtrC是介导电子转移到细胞外基质的细胞外元素，而MtrB是跨鞘的跨-OM蛋白，充当鞘，MtrA和MtrC在其中交换电子。我们已经在一系列细菌门中鉴定了MtrAB模块，这表明它被广泛用于与细胞外环境进行电子交换。

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  • 作者

    Robert S. Hartshome; Catherine L. Reardon; Daniel Ross; Jochen Nuester; Thomas A. Clarke; Andrew J. Gates; Paul C. Mills; Jim K. Fredrickson; John M. Zachara; Liang Shi; Alex S. Beliaev; Matthew J. Marshall; Ming Tien; Susan Brantley; Julea N. Butt; David J. Richardson;

  • 作者单位

    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom;

    Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352;

    Departments of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802-4500;

    Departments of Geoscience, Pennsylvania State University, University Park, PA 16802-4500;

    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom;

    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom;

    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom;

    Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352;

    Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352;

    Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352;

    Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352;

    Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352;

    Departments of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802-4500;

    Departments of Geoscience, Pennsylvania State University, University Park, PA 16802-4500;

    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom;

    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cytochrome-c; iron respiration; protein film voltammetry electron paramagnetic resonance; Shewanella;

    机译：细胞色素c铁呼吸蛋白质膜伏安法电子顺磁共振希瓦内拉;

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