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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Uncoupling of sustained MAMP receptor signaling from early outputs in an Arabidopsis endoplasmic reticulum glucosidase II allele
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Uncoupling of sustained MAMP receptor signaling from early outputs in an Arabidopsis endoplasmic reticulum glucosidase II allele

机译：拟南芥内质网葡糖苷酶II等位基因的早期输出与持续的MAMP受体信号解偶联

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摘要

Recognition of microbe-associated molecular patterns (MAMPs), conserved structures typical of a microbial class, triggers immune responses in eukaryotes. This is accompanied by a diverse set of physiological responses that are thought to enhance defense activity in plants. However, the extent and mechanisms by which MAMP-induced events contribute to host immunity are poorly understood. Here we reveal Arabidopsis priority in sweet Iife4 (psl4) and psl5 mutants that are insensitive to the bacterial elongation factor (EF)-Tu epitope elf 18 but responsive to flagellin epitope fIg22. PSL4 and PSL5, respectively, identify β- and α-subunits of endoplasmic reticulum-resident glucosidase II, which is essential for stable accumulation and quality control of the elf18 receptor EFR but not the flg22 receptor FLS2. We notice that EFR signaling is partially and differentially impaired without a significant decrease of the receptor steady-state levels in 2 weakly dysfunctional gllα alleles, designated psl5-1 and rsw3. Remarkably, rsw3 plants exhibit marked supersusceptibility against a virulent bacterial phytopathogen despite nearly intact coactivation of MAPKs, reactive oxygen species, ethylene biosynthesis, and callose deposition in response to elf18, demonstrating that these signaling outputs alone are insufficient to mount effective immunity. However, rsw3 plants fail to maintain high transcript levels of defense-promoting WRKY, PR1, and PR2 genes at late time points (4 to 24 h) after elf18 elicitation. This points to an unexpected separation between initial and sustained activation of EFR-mediated signaling in the absence of proper glucosidase li-mediated endoplasmic reticulum quality control. Our findings strongly suggest the importance of sustained MAMP receptor signaling as a key step in the establishment of robust immunity.
机译：微生物相关分子模式（MAMP）的识别是微生物类别的典型保守结构，可触发真核生物的免疫反应。这伴随着各种各样的生理反应，这些反应被认为可以增强植物的防御活性。但是，对MAMP诱导的事件有助于宿主免疫的程度和机制了解甚少。在这里，我们揭示了甜Iife4（psl4）和psl5突变体中的拟南芥优先级，这些突变体对细菌延伸因子（EF）-Tu表位小精灵18不敏感，但对鞭毛蛋白表位fIg22有反应。 PSL4和PSL5分别识别内质网驻留型葡糖苷酶II的β和α亚基，这对于elf18受体EFR而非flg22受体FLS2的稳定积累和质量控制至关重要。我们注意到，在2个弱功能障碍的gllα等位基因（分别称为psl5-1和rsw3）中，EFR信号传导受到部分和差异性破坏，而受体稳态水平没有显着降低。值得注意的是，rsw3植物显示出对强力细菌性植物病原体的明显超敏感性，尽管MAPK，活性氧，乙烯生物合成和响应elf18的call质沉积几乎完好无损地共激活，表明仅这些信号输出不足以提高有效免疫力。但是，rsw3植物在elf18诱导后的较晚时间点（4至24小时）无法保持高水平的防御防御性WRKY，PR1和PR2基因转录水平。这表明在缺乏适当的葡萄糖苷酶li介导的内质网质量控制的情况下，EFR介导的信号的初始激活和持续激活之间存在意想不到的分离。我们的发现强烈表明，持续建立MAMP受体信号作为建立强大免疫力的关键步骤的重要性。

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  • 作者

    Xunli Lu; Nico Tintor; Tobias Mentzel; Erich Kombrink; Thomas Boller; Silke Robatzek; Paul Schulze-Lefert; Yusuke Saijo;

  • 作者单位

    Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany;

    Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany;

    Botanical Institute, University of Basel, 4056 Basel, Switzerland;

    Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany;

    Botanical Institute, University of Basel, 4056 Basel, Switzerland;

    Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany;

    Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany;

    Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    EFR; ER quality control; LRR RLK; plant immunity;

    机译：EFR;ER质量控制;LRR RLK;植物免疫;

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