...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Mammalian acyl-CoA:lysophosphatidylcholine acyltransferase enzymes
【24h】

Mammalian acyl-CoA:lysophosphatidylcholine acyltransferase enzymes

机译：哺乳动物酰基辅酶A：溶血磷脂酰胆碱酰基转移酶

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The mammalian RBC lacks de novo lipid synthesis but maintains its membrane composition by rapid turnover of acyl moieties at the sn-2 position of phospholipids. Plasma-derived fatty acids are esterified to acyl-CoA by acyl-CoA synthetases and transferred to lysophospho-lipids by acyl-CoA:lysophospholipid acyltransferases. We report the characterization of three lysophosphatidylcholine (lysoPC) acyltransferases (LPCATs), products of the AYTL1, -2, and -3 genes. These proteins are three members of a LPCAT family, of which all three genes are expressed in an erythroleukemic cell line. Aytl2 mRNA was detected in mouse reticulocytes, and the presence of the product of the human ortholog was confirmed in adult human RBCs. The three murine Aytl proteins generated phosphatidylcholine from long-chain acyl-CoA and lysoPC when expressed in Escherichia coli membranes. Spliced variants of Aytl1, affecting a conserved catalytic motif, were identified. Calcium and magnesium modulated LPCAT activity of both AytH and -2 proteins that exhibit EF-hand motifs at the C terminus. Characterization of the product of the Aytl2 gene as the phosphatidylcholine reacylating enzyme in RBCs represents the identification of a plasma membrane lysophospholipid acyltransferase and establishes the function of a LPCAT protein.
机译：哺乳动物RBC缺乏从头合成脂质，但通过磷脂在sn-2位置上的酰基部分快速转换来维持其膜组成。血浆来源的脂肪酸通过酰基-CoA合成酶酯化为酰基-CoA，并通过酰基-CoA：溶血磷脂酰基转移酶转移到溶血磷脂。我们报告了三种溶血磷脂酰胆碱（lysoPC）酰基转移酶（LPCATs），AYTL1，-2和-3基因产物的表征。这些蛋白质是LPCAT家族的三个成员，其中所有三个基因均在红白血病细胞系中表达。在小鼠网织红细胞中检测到Aytl2 mRNA，并且在成年人类RBC中证实了人类直系同源物产物的存在。当在大肠杆菌膜中表达时，三种鼠类Aytl蛋白从长链酰基辅酶A和lysoPC生成磷脂酰胆碱。确定了影响保守催化基序的Aytl1的剪接变体。钙和镁调节AytH和-2蛋白的LPCAT活性，这些蛋白在C端显示EF-手基序。 Aytl2基因产物作为RBC中的磷脂酰胆碱再酰化酶的表征代表了质膜溶血磷脂酰基转移酶的鉴定，并建立了LPCAT蛋白的功能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号