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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea
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The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea

机译：细胞壁的分解，成熟和对灰葡萄孢的果实敏感性之间的交集

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摘要

Fruit ripening is characterized by processes that modify texture and flavor but also by a dramatic increase in susceptibility to necrotrophic pathogens, such as Botrytis cinerea. Disassembly of the major structural polysaccharides of the cell wall (CW) is a significant process associated with ripening and contributes to fruit softening. In tomato, polygalacturonase (PG) and expansin (Exp) are among the CW proteins that cooperatively participate in ripening-associated CW disassembly. To determine whether endogenous CW disassembly influences the ripening-regulated increase in necrotropic pathogen susceptibility, B. cinerea susceptibility was assessed in transgenic fruit with suppressed polygalacturonase (LePG) and expansin (LeExp1) expression. Suppression of either LePG or LeExp1 alone did not reduce susceptibility but simultaneous suppression of both dramatically reduced the susceptibility of ripening fruit to B. cinerea, as measured by fungal biomass accumulation and by macerating lesion development. These results demonstrate that altering endogenous plant CW disassembly during ripening influences the course of infection by B. cinerea, perhaps by changing the structure or the accessibility of CW substrates to pathogen CW-degrading enzymes. Recognition of the role of ripening-associated CW metabolism in postharvest pathogen susceptibility may be useful in the design and development of strategies to limit pathogen losses during fruit storage, handling, and distribution.
机译：果实成熟的特征在于改变质地和风味的过程，但其特征还在于对坏死性病原体（如灰葡萄孢）的易感性急剧增加。细胞壁（CW）的主要结构多糖的拆卸是与成熟相关的重要过程，并有助于果实软化。在番茄中，聚半乳糖醛酸酶（PG）和扩展酶（Exp）是协同参与成熟相关的CW分解的CW蛋白。为了确定内源性CW分解是否影响促生性病原体易感性的成熟调控增加，在多聚半乳糖醛酸酶（LePG）和弹性蛋白（LeExp1）表达受到抑制的转基因水果中评估了灰葡萄孢的易感性。单独抑制LePG或LeExp1并不能降低药敏性，但同时抑制两者都可以显着降低成熟果实对灰葡萄孢的敏感性，这是通过真菌生物量积累和浸软性病变发展来衡量的。这些结果表明，在成熟过程中改变内源植物CW的分解可能会影响灰质芽孢杆菌的感染过程，可能是通过改变CW底物对病原体CW降解酶的可达性或可及性来实现的。认识到与成熟相关的CW代谢在收获后病原体易感性中的作用可能有助于设计和开发策略，以限制水果在贮藏，处理和分配过程中病原体的损失。

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