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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Micromechanical properties of keratin intermediate filament networks
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Micromechanical properties of keratin intermediate filament networks

机译：角蛋白中间长丝网络的微机械性能

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摘要

Keratin intermediate filaments (KIFs) form cytoskeletal KIF networks that are essential for the structural integrity of epithelial cells. However, the mechanical properties of the in situ network have not been defined. Particle-tracking microrheology (PTM) was used to obtain the micromechanical properties of the KIF network in alveolar epithelial cells (AECs), independent of other cytoskeletal components, such as microtubules and microfilaments. The storage modulus (G') at 1 Hz of the KIF network decreases from the perinuclear region (335 dyn/cm~2) to the cell periphery (95 dyn/cm~2), yielding a mean value of 210 dyn/cm~2. These changes in 6' are inversely proportional to the mesh size of the network, which increases 10-fold from the perinuclear region (0.02 μm~2) to the cell periphery (0.3 μm~2). Shear stress (15 dyn/cm~2 for 4 h) applied across the surface of AECs induces a more uniform distribution of KIF, with the mesh size of the network ranging from 0.02 μm~2 near the nucleus to only 0.04 μm~2 at the cell periphery. This amounts to a 40% increase in the mean G'. The storage modulus of the KIF network in the perinuclear region accurately predicts the shear-induced deflection of the cell nucleus to be 0.87 ± 0.03 μm. The high storage modulus of the KIF network, coupled with its solid-like rheological behavior, supports the role of KIF as an intracellular structural scaffold that helps epithelial cells to withstand external mechanical forces.
机译：角蛋白中间丝（KIF）形成细胞骨架KIF网络，这对于上皮细胞的结构完整性至关重要。但是，尚未定义原位网络的机械性能。颗粒追踪微流变学（PTM）用于获得肺泡上皮细胞（AEC）中KIF网络的微机械特性，而与其他细胞骨架成分（例如微管和微丝）无关。 KIF网络在1 Hz处的储能模量（G'）从核周围区域（335 dyn / cm〜2）到细胞周围（95 dyn / cm〜2）减小，平均值为210 dyn / cm〜。 2。 6'的这些变化与网络的网眼大小成反比，网络的网眼大小从核周区域（0.02μm〜2）到细胞周围（0.3μm〜2）增加10倍。施加在AEC表面的剪应力（15 dyn / cm〜2持续4 h）导致KIF分布更均匀，网络的网眼大小从靠近原子核的0.02μm〜2到仅在原子核附近的0.04μm〜2细胞外围。这相当于平均G'增加了40％。 KIF网络在核周区域中的储能模量准确地预测了剪切诱导的细胞核偏转为0.87±0.03μm。 KIF网络的高储能模量，加上其类似固体的流变行为，支持了KIF作为细胞内结构支架的作用，该结构支架有助于上皮细胞承受外部机械力。

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