...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures
【24h】

Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures

机译：选择性铝钝化可将单个DNA聚合酶分子靶向固定在零模波导纳米结构中

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Optical nanostructures have enabled the creation of subdiffraction detection volumes for single-molecule fluorescence microscopy. Their applicability is extended by the ability to place molecules in the confined observation volume without interfering with their biological function. Here, we demonstrate that processive DNA synthesis thousands of bases in length was carried out by individual DNA polymerase molecules immobilized in the observation volumes of zero-mode waveguides (ZMWs) in high-density arrays. Selective immobilization of polymerase to the fused silica floor of the ZMW was achieved by passivation of the metal cladding surface using polyphosphonate chemistry, producing enzyme density contrasts of glass over aluminum in excess of 400:1. Yields of single-molecule occupancies of ≈30% were obtained for a range of ZMW diameters (70-100 nm). Results presented here support the application of immobilized single DNA polymerases in ZMW arrays for long-read-length DNA sequencing.
机译：光学纳米结构使得能够为单分子荧光显微镜创建亚衍射检测体积。通过将分子放置在有限的观察空间而不干扰其生物学功能的能力，扩展了它们的适用性。在这里，我们证明了由固定在高密度阵列中零模波导（ZMWs）的观察体积中的单个DNA聚合酶分子进行了长度为数千个碱基的过程性DNA合成。通过使用聚膦酸酯化学方法钝化金属覆层表面，将聚合酶选择性固定在ZMW的熔融石英地板上，从而在铝上产生超过400：1的玻璃与铝的酶密度对比。对于一系列ZMW直径（70-100 nm），单分子占有率约为30％。此处提供的结果支持固定化的单个DNA聚合酶在ZMW阵列中用于长读长度DNA测序的应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号