• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Interaction between HMGAIa and the origin recognition complex creates site-specific replication origins
【24h】

Interaction between HMGAIa and the origin recognition complex creates site-specific replication origins

机译：HMGAIa与起源识别复合体之间的相互作用创建特定于站点的复制起源

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In all eukaryotic cells, origins of DNA replication are characterized by the binding of the origin recognition complex (ORC). How ORC is positioned to sites where replication initiates is unknown, because metazoan ORC binds DNA without apparent sequence specificity. Thus, additional factors might be involved in ORC positioning. Our experiments indicate that a family member of the high-mobility group proteins, HMGAIa, can specifically target ORC to DNA. Coimmunoprecipitations and imaging studies demonstrate that HMGAIa interacts with different ORC subunits in vitro and in vivo. This interaction occurs mainly in AT-rich heterochro-matic regions to which HMGAIa localizes. Fusion proteins of HMGAIa and the DNA-binding domain of the viral factor EBNA1 or the prokaryotic tetracycline repressor, TetR, can recruit ORC to cognate operator sites forming functional origins of DNA replication. When HMGAIa is targeted to plasmid DNA, the prereplicative complex is assembled during G_1 and the amount of ORC correlates with the local concentration of HMGAIa. Nascent-strand abundance assays demonstrate that DNA replication initiates at or near HMGA1a-rich sites. Our experiments indicate that chromatin proteins can target ORC to DNA, suggesting they might specify origins of DNA replication in metazoan cells.
机译：在所有的真核细胞中，DNA复制的起点都以起点识别复合物（ORC）的结合为特征。由于后生动物ORC结合DNA时没有明显的序列特异性，因此ORC如何定位于复制开始的位置。因此，ORC定位中可能涉及其他因素。我们的实验表明，高迁移率族蛋白HMGAIa的家族成员可以特异性地将ORC靶向DNA。免疫共沉淀和成像研究表明，HMGAIA在体外和体内与不同的ORC亚基相互作用。这种相互作用主要发生在HMGAIa所在的富含AT的异质区域中。 HMGAIa和病毒因子EBNA1的DNA结合结构域或原核四环素阻遏物TetR的融合蛋白可以募集ORC来关联形成DNA复制功能起点的操纵位点。当HMGAIa靶向质粒DNA时，复制前复合物在G_1期间组装，并且ORC的量与HMGAIa的局部浓度相关。新生链丰度测定表明，DNA复制起始于富含HMGA1a的位点或附近。我们的实验表明，染色质蛋白可以将ORC靶向DNA，表明它们可能指定后生细胞中DNA复制的起源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号