...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae
【24h】

Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae

机译：Pier蝶科蝴蝶粗提物中细胞毒性和DNA ADP-核糖基化活性的分布

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cabbage butterflies, Pieris rapae and Pieris brassicae, contain strong cytotoxic proteins, designated as pierisin-1 and -2, against cancer cell lines. These proteins exhibit DNA ADP-ribosylating activity. To determine the distribution of substances with cytotox-icity and DNA ADP-ribosylating activity among other species, crude extracts from 20 species of the family Pieridae were examined for cytotoxicity in HeLa cells and DNA ADP-ribosylating activity. Both activities were detected in extracts from 13 species: subtribes Pierina (Pieris rapae, Pieris canidia, Pieris napi, Pieris melete, Pieris brassicae, Pontia daplidice, and Talbotia naganum), Aporiina (Apo-ria gigantea, Aporia crataegi, Aporia hippia, and Delias pasithoe), and Appiadina (Appias nero and Appias paulina). All of these extracts contained substances recognized by anti-pierisin-1 antibodies, with a molecular mass of ≈100 kDa established earlier for pierisin-1. Moreover, sequences containing NAD-binding sites, conserved in ADP-ribosyltransferases, were amplified from genomic DNA from 13 species of butterflies with cytotoxicity and DNA ADP-ribosylating activity by PCR. Extracts from seven species, Appias lyncida, Leptosia nina, Anthocharis scolymus, Eurema he-cabe, Catopsilia pomona, Catopsilia scylla, and Colias erate, showed neither cytotoxicity nor DNA ADP-ribosylating activity, and did not contain substances recognized by anti-pierisin-1 antibodies. Sequences containing NAD-binding sites were not amplified from genomic DNA from these seven species. Thus, pierisin-like proteins, showing cytotoxicity and DNA ADP-ribosylating activity, are suggested to be present in the extracts from butterflies not only among the subtribe Pierina, but also among the subtribes Aporiina and Appiadina. These findings offer insight to understanding the nature of DNA ADP-ribosylating activity in the butterfly.
机译：白菜蝶，菜青虫和菜青虫均含有针对癌细胞系的强细胞毒性蛋白，分别命名为Pierisin-1和-2。这些蛋白质表现出DNA ADP-核糖基化活性。为了确定具有细胞毒性和DNA ADP核糖基化活性的物质在其他物种中的分布，检查了来自Pieridae科20种物种的粗提物对HeLa细胞的细胞毒性和DNA ADP核糖基化活性。两种活性均在以下13种物种的提取物中检测到：亚皮亚属（Pieris rapae，皮埃斯canidia，Pieris napi，Pieris melete，Pieris Brasicae，Pontia daplidice和Talbotia naganum），Apooriina（Apo-ria gigantea，Aporia crataegi，Aporia hippia和Delias pasithoe）和Appiadina（Appias nero和Appias paulina）。所有这些提取物均含有抗抗pierisin-1抗体识别的物质，其分子量为pierisin-1较早建立的≈100kDa。此外，通过PCR从13种蝴蝶的基因组DNA中扩增了含有ADP-核糖基转移酶中保守的NAD结合位点的序列，具有细胞毒性和DNA ADP-核糖基化活性。七种动物的提取物，即Appias lyncida，Leptosia nina，Anthocharis scolymus，Eurema he-cabe，Catopsilia pomona，Catopsilia scylla和Colias erate，均未显示出细胞毒性或DNA ADP核糖基化活性，并且不含抗皮埃希斯菌素识别的物质1抗体。没有从这七个物种的基因组DNA中扩增出含有NAD结合位点的序列。因此，表明不仅在皮埃里纳（Pierina）亚族中，而且在Apooriina和Appiadina亚族中，从蝴蝶提取物中也存在表现出细胞毒性和DNA ADP-核糖基化活性的类脂蛋白样蛋白。这些发现为了解蝴蝶中DNA ADP-核糖基化活性的性质提供了见识。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号