Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences

Eirik W. Lundblad; Gaoping Xiao; Jae-hyeong Ko; Sidney Altman

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Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences

机译：大肠杆菌RNase P和随机外部引导序列可快速选择RNA中可及和可裂解的位点

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A method of inhibiting the expression of particular genes by using external guide sequences (EGSs) has been improved in its rapidity and specificity. Random EGSs that have 14-nt random sequences are used in the selection procedure for an EGS that attacks the mRNA for a gene in a particular location. A mixture of the random EGSs, the particular target RNA, and RNase P is used in the diagnostic procedure, which, after completion, is analyzed in a gel with suitable control lanes. Within a few hours, the procedure is complete. The action of EGSs designed by an older method is compared with EGSs designed by the random EGS method on mRNAs from two bacterial pathogens.

机译：通过使用外部引导序列（EGS）抑制特定基因表达的方法，其速度和特异性得到了改善。在攻击特定位置的基因的mRNA的EGS的选择程序中，使用具有14个核苷酸随机序列的随机EGS。在诊断过程中使用随机EGS，特定靶标RNA和RNase P的混合物，完成后，在带有合适对照泳道的凝胶中进行分析。在几个小时内，该过程完成。比较了用较旧方法设计的EGS与用随机EGS方法设计的EGS对两种细菌病原体mRNA的作用。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2008年第7期|p.2354-2357|共4页
作者
Eirik W. Lundblad; Gaoping Xiao; Jae-hyeong Ko; Sidney Altman;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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机译：外部指导序列和RNase P抑制鼠疫耶尔森菌的毒力基因表达。
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6. Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences [O] . Eirik W. Lundblad, Gaoping Xiao, Jae-hyeong Ko, 2008

机译：大肠杆菌RNase P和随机外部引导序列可快速选择RNA中可及和可裂解的位点
7. Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences [O] . Lundblad, Eirik W., Xiao, Gaoping, Ko, Jae-hyeong, 2008

机译：大肠杆菌RNase P和随机外部引导序列可快速选择RNA中可及和可裂解的位点

Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences

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