Temperature and pressure dependence of protein stability: The engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram

Johannes Wiedersich; Simone Koehler; Arne Skerra; Josef Friedrich

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Temperature and pressure dependence of protein stability: The engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram

机译：温度和压力对蛋白质稳定性的依赖性：工程改造的荧光素结合脂钙素FluA显示椭圆相图

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摘要

We have measured the equilibrium constant for the denaturation transition of the engineered fluorescein-binding lipocalin FluA as a function of pressure and temperature, taking advantage of the fact that the ligand's fluorescence is almost fully quenched

机译：我们利用配体的荧光几乎完全淬灭的事实，测量了工程化的荧光素结合脂环蛋白FluA的变性转变的平衡常数与压力和温度的关系。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2008年第15期|5756-5761|共6页
作者
Johannes Wiedersich; Simone Koehler; Arne Skerra; Josef Friedrich;
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作者单位

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
anticalin; denaturation; engineering; folding; thermodynamics;

机译：安替林变性;工程;折叠热力学;

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5. Pressure-temperature phase diagram of tris(hydroxymethyl)aminomethane and phase diagram determination of tris(hydroxymethyl)aminomethane -- 2-amino-2-methyl-1, 3-propanediol (AMPL) binary system. [D] . Kamisetty, Vamsi Krishna. 2010

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6. Temperature and pressure dependence of protein stability: The engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram [O] . Johannes Wiedersich, Simone Köhler, Arne Skerra, 2008

机译：温度和压力对蛋白质稳定性的依赖性：工程改造的荧光素结合脂钙素FluA显示椭圆相图
7. Temperature and pressure dependence of protein stability: The engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram [O] . Wiedersich, Johannes, Köhler, Simone, Skerra, Arne, 2008

机译：温度和压力对蛋白质稳定性的依赖性：工程改造的荧光素结合脂钙素FluA显示椭圆相图

Temperature and pressure dependence of protein stability: The engineered fluorescein-binding lipocalin FluA shows an elliptic phase diagram

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