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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Chiral Discrimination Of The Japanese Beetle Sex Pheromone And A Behavioral Antagonist By A Pheromone-degrading Enzyme
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Chiral Discrimination Of The Japanese Beetle Sex Pheromone And A Behavioral Antagonist By A Pheromone-degrading Enzyme

机译：信息素降解酶对日本甲虫性信息素和行为拮抗剂的手性区分

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摘要

The sophistication of the insect olfactory system is elegantly demonstrated by the reception of sex pheromone by the Japanese beetle. In this insect, two olfactory receptor neurons housed in antennal sensilla placodea are highly sensitive. One neuron specifically detects the sex pheromone produced by conspecific females (R,Z)-5-(-)-(1-decenyl)oxacyclopentan-2-one [(R)-japonil-ure]. The other neuron is tuned to (S)-japonilure, a sex pheromone from a closely related species and a behavioral antagonist for the Japanese beetle. These chemical signals are enzymatically terminated by antennal esterases that open the lactone rings to form physiologically inactive hydroxyacids. We have isolated a pheromone-degrading enzyme, PjapPDE, from > 100,000 antennae of the Japanese beetle. PjapPDE was demonstrated to be expressed only in the antennal tissues housing the pheromone-detecting sensilla placodea. Baculovirus expression generated recombinant PjapPDE with likely the same posttranslational modifications as the native enzyme. Kinetic studies with pure native and recombinant PjapPDE showed a clear substrate preference, with an estimated half-life in vivo for the sex pheromone and a behavioral antagonist of ≈ 30 and ≈ 90 ms, respectively.
机译：日本甲虫对性信息素的接受优雅地证明了昆虫嗅觉系统的先进性。在这只昆虫中，触角感器中的两个嗅觉感受器神经元高度敏感。一种神经元特异性地检测由同种雌性（R，Z）-5-（-）-（1-癸烯基）氧环环戊烷-2-one [[R] -japonil-ure]产生的性信息素。另一个神经元被调整为（S）-japonilure，一种来自密切相关物种的性信息素，是日本甲虫的行为拮抗剂。这些化学信号被触角酯酶酶促终止，触角酯酶打开内酯环形成生理上无活性的羟基酸。我们从日本甲虫的100,000多根触角中分离出了一种信息素降解酶PjapPDE。 PjapPDE被证明仅在容纳信息素的感官placodea的触角组织中表达。杆状病毒表达产生了重组PjapPDE，其翻译后修饰可能与天然酶相同。使用纯天然PjapPDE和重组PjapPDE进行的动力学研究显示出明显的底物偏爱，性信息素和行为拮抗剂的体内估计半衰期分别为≈30 ms和≈90 ms。

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