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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Conformational flexibility facilitates self-assembly of complex DNA nanostructures
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Conformational flexibility facilitates self-assembly of complex DNA nanostructures

机译：构象灵活性有助于复杂DNA纳米结构的自组装

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摘要

Molecular self-assembly is a promising approach to the preparation of nanostructures. DNA, in particular, shows great potential to be a superb molecular system. Synthetic DNA molecules have been programmed to assemble into a wide range of nanostructures. It is generally believed that rigidities of DNA nanomotifs (tiles) are essential for programmable self-assembly of well defined nanostructures. Recently, we have shown that adequate conformational flexibility could be exploited for assembling 3D objects, including tetrahedra, dodecahedra, and buckyballs, out of DNA three-point star motifs. In the current study, we have integrated tensegrity principle into this concept to assemble well defined, complex nanostructures in both 2D and 3D. A symmetric five-point-star motif (tile) has been designed to assemble into icosahedra or large nanocages depending on the concentration and flexibility of the DNA tiles. In both cases, the DNA tiles exhibit significant flexibilities and undergo substantial conformational changes, either symmetrically bending out of the plane or asymmetrically bending in the plane. In contrast to the complicated natures of the assembled structures, the approach presented here is simple and only requires three different component DNA strands. These results demonstrate that conformational flexibility could be explored to generate complex DNA nanostructures. The basic concept might be further extended to other biomacromolecular systems, such as RNA and proteins.
机译：分子自组装是制备纳米结构的一种有前途的方法。尤其是DNA，它显示出极好的潜力，成为了一个极好的分子系统。合成的DNA分子已被编程为组装成各种纳米结构。通常认为，DNA纳米基序（切片）的刚性对于定义良好的纳米结构的可编程自组装至关重要。最近，我们已经证明可以利用足够的构象灵活性从DNA三点星形图案中组装3D对象，包括四面体，十二面体和布基球。在当前的研究中，我们已将张力原理整合到此概念中，以在2D和3D中组装定义明确的复杂纳米结构。根据DNA瓷砖的浓度和柔韧性，已经设计了对称的五点星形图案（tile）组装成二十面体或大型纳米笼。在这两种情况下，DNA贴片都显示出显着的柔韧性，并且经历了显着的构象变化，即对称地弯曲出平面或不对称地弯曲在平面中。与组装结构的复杂性质相反，此处介绍的方法很简单，只需要三个不同的成分DNA链即可。这些结果证明，可以探索构象柔性来产生复杂的DNA纳米结构。基本概念可能会进一步扩展到其他生物大分子系统，例如RNA和蛋白质。

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