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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Transmembrane domain length of viral K~+ channels is a signal for mitochondria targeting
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Transmembrane domain length of viral K~+ channels is a signal for mitochondria targeting

机译：病毒K〜+通道的跨膜结构域长度是线粒体靶向的信号

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摘要

K~+ channels operate in the plasma membrane and in membranes of organelles including mitochondria. The mechanisms and topo-genlc information for their differential synthesis and targeting is unknown. This article describes 2 similar viral K~+ channels that are differentially sorted; one protein (Kesv) is imported by the Tomrncomplex into the mitochondria, the other (Kcv) to the plasmarnmembrane. By creating chimeras we discovered that mitochondrial sorting of Kesv depends on a hierarchical combination of N- andrnC-terminal signals. Crucial is the length of the second transmembrane domain; extending its C terminus by ≥2 hydrophobic amino acids redirects Kesv from the mitochondrial to the plasma membrane. Activity of Kesv in the plasma membrane is detected electrically or by yeast rescue assays only after this shift in sorting. Hence only minor structural alterations in a transmembrane domain are sufficient to switch sorting of a K~+ channel between the plasma membrane and mitochondria.
机译：钾离子通道在质膜和包括线粒体在内的细胞器膜中起作用。其差异合成和靶向的机制和拓扑基因信息尚不清楚。本文介绍了2个相似的病毒K〜+通道，它们进行了分选。 Tomrncomplex将一种蛋白（Kesv）通过线粒体导入，将另一种蛋白（Kcv）导入血浆膜。通过创建嵌合体，我们发现Kesv的线粒体分选取决于N端和rnC端信号的层次组合。关键是第二个跨膜结构域的长度；将C末端延伸≥2个疏水氨基酸，将Kesv从线粒体重定向到质膜。仅在分选发生这种变化之后，才能通过电或酵母拯救检测法检测质膜中的Kesv活性。因此，仅跨膜结构域的微小结构改变足以切换质膜和线粒体之间的K +通道的分类。

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