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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Noninvasive tracking of gene expression by reporter transgene imaging
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Noninvasive tracking of gene expression by reporter transgene imaging

机译：通过记者转基因成像无创跟踪基因表达

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摘要

In this issue of PNAS, the laboratory that discovered leptin (1) reports the development of a novel in vivo imaging strategy that permits noninvasive estimation of leptin expression (2). They have engineered a BAC transgenic mouse strain expressing a luciferase-reporter gene under the control of adipocyte-specific leptin regulatory sequences. Because of the ample evidence of correlation between lipid storage and leptin (3, 4), they reasoned that a change in adipocyte mass would be accompanied by a parallel change in the expression of endogenous leptin and of the luciferase transgene. This was, in fact, the case. They were able to show in vivo differences in the adipocyte images of fed, fasting, and ob/ob transgenic animals. Luciferase activity fell gradually during a 3-day fast, but returned to baseline levels within 6 h of refeeding. Similarly, lipid depletion by infusion of recombinant leptin depleted adipocyte fat and, on discontinuation of leptin treatment, luciferase expression returned toward normal. Thus, in these two models, leptin expression as measured by the luciferase imaging is highly correlated with adipose tissue mass and can even be used as a surrogate for its measurement.
机译：在本期PNAS中，发现瘦素的实验室（1）报告了一种新的体内成像策略的发展，该策略可无创地评估瘦蛋白的表达（2）。他们设计了在脂肪细胞特异性瘦素调节序列控制下表达荧光素酶报道基因的BAC转基因小鼠品系。由于有充分的证据表明脂质存储与瘦素之间存在相关性（3、4），他们认为脂肪细胞量的变化将伴随着内源性瘦素和荧光素酶转基因表达的平行变化。实际上就是这种情况。他们能够在喂养，禁食和ob / ob转基因动物的脂肪细胞图像中显示体内差异。萤光素酶活性在3天禁食中逐渐下降，但在重新喂食后6小时内恢复到基线水平。类似地，通过输注重组瘦蛋白消耗的脂质减少了脂肪细胞的脂肪，并且在瘦素治疗中断后，荧光素酶的表达恢复了正常。因此，在这两个模型中，通过萤光素酶成像测量的瘦素表达与脂肪组织质量高度相关，甚至可以用作其测量的替代物。

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