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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development
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Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development

机译:含水稻jmjC结构域的基因JMJ706编码花器官发育所需的H3K9脱甲基酶

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摘要

Histone lysine methylation is an important epigenetic modification with both activating and repressive roles in gene expression. Jumonji C (jmjC) domain-containing proteins have been shown to reverse histone methylation in nonplant model systems. Here, we show that plant Jumonji C proteins have both conserved and specific features compared with mammalian homologues. In particular, the rice JMJD2 family jmjC gene JMJ706 is shown to encode a heterochromatin-enriched protein. The JMJ706 protein specifically reverses di- and trimethylations of lysine 9 of histone H3 (H3K9) in vitro. Loss-of-function mutations of the gene lead to increased di- and trimethylations of H3K9 and affect the spikelet development, including altered floral morphology and organ number. Gene expression and histone modification analysis indicates that JMJ706 regulates a subset of flower development regulatory genes. Taken together, our data suggest that rice JMJ706 encodes a heterochromatin-associated H3K9 demethylase involved in the regulation of flower development in rice.
机译:组蛋白赖氨酸甲基化是重要的表观遗传修饰,在基因表达中具有激活和抑制作用。在非植物模型系统中,含有Jumonji C(jmjC)域的蛋白质已显示可逆转组蛋白甲基化。在这里,我们显示与哺乳动物同系物相比,植物Jumonji C蛋白既具有保守的特征又具有特定的特征。特别地,水稻JMJD2家族jmjC基因JMJ706显示编码富含异染色质的蛋白质。 JMJ706蛋白在体外特异性逆转组蛋白H3(H3K9)赖氨酸9的二甲基和三甲基化。该基因的功能丧失突变导致H3K9的二甲基和三甲基化增加,并影响小穗发育,包括改变的花形和器官数量。基因表达和组蛋白修饰分析表明,JMJ706调节花发育调节基因的一个子集。两者合计,我们的数据表明水稻JMJ706编码与染色质相关的H3K9脱甲基酶,参与水稻花发育的调控。

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