...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Distinct size distribution of endogeneous siRNAs in maize: Evidence from deep sequencing in the mop1-1 mutant
【24h】

Distinct size distribution of endogeneous siRNAs in maize: Evidence from deep sequencing in the mop1-1 mutant

机译：玉米中内源性siRNA的独特大小分布：来自mop1-1突变体的深度测序的证据

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Small RNAs from plants are known to be highly complex and abundant, with this complexity proportional to genome size. Most endogenous siRNAs in Arabidopsis are dependent on RNA-DEPEN-DENTRNA POLYMERASE2 (RDR2) for their biogenesis. Recent work has demonstrated that the maize MEDIATOR OFPARAMUTATION1 (mop1) gene is a predicted ortholog of RDR2. The mop1 gene is required for establishment of paramutation and maintenance of transcriptional silencing of transposons and transgenes, suggesting the potential involvement of small RNAs. We analyzed small RNAs in wild-type maize and in the isogenic mop1-1 loss-of-function mutant by using Illumina's sequencing-by-synthesis (SBS) technology, which allowed us to characterize the complement of maize small RNAs to considerable depth. Similar to rdr2 in Arabidopsis, in mop1-1, the 24-nucleotide (nt) endogenous heterochro-matic short-interfering siRNAs were dramatically reduced, resulting in an enrichment of miRNAs and transacting siRNAs. In contrast to the Arabidopsis rdr2 mutant, the mop1-1 plants retained a highly abundant heterochromatic 22-nt class of small RNAs, suggesting a second mechanism for heterochromatic siRNA production. The enrichment of miRNAs and loss of 24-nt heterochromatic siRNAs in mop1-1 should be advantageous for miRNA discovery as the maize genome becomes more fully sequenced.
机译：已知来自植物的小RNA非常复杂和丰富，这种复杂性与基因组大小成正比。拟南芥中的大多数内源性siRNA的生物发生都依赖于RNA-DEPEN-DENTRNA POLYMERASE2（RDR2）。最近的工作表明，玉米介体的传代机制（mop1）基因是RDR2的预测直系同源物。 mop1基因是建立突变和维持转座子和转基因的转录沉默所必需的，这表明小RNA的潜在参与。通过使用Illumina的合成测序（SBS）技术，我们分析了野生型玉米和等基因mop1-1功能丧失突变体中的小RNA，这使我们能够将玉米小RNA的互补序列特征化。与拟南芥中的rdr2相似，在mop1-1中，24核苷酸（nt）内源性异源短干扰siRNA显着减少，导致miRNA富集和交易性siRNA。与拟南芥rdr2突变体相反，mop1-1植物保留了高度丰富的异色22-nt类小RNA，这提示了产生异色siRNA的第二种机制。随着玉米基因组序列变得更完整，mop1-1中miRNA的富集和24 nt异色siRNA的丢失应有利于miRNA的发现。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号