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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Production Of Healthy Cloned Mice From Bodies Frozen At -20℃ For 16 Years
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Production Of Healthy Cloned Mice From Bodies Frozen At -20℃ For 16 Years

机译：用-20℃冷冻16年的尸体生产健康的克隆小鼠

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摘要

Cloning animals by nuclear transfer provides an opportunity to preserve endangered mammalian species. However, it has been suggested that the "resurrection" of frozen extinct species (such as the woolly mammoth) is impracticable, as no live cells are available, and the genomic material that remains is inevitably degraded. Here we report production of cloned mice from bodies kept frozen at -20 ℃ for up to 16 years without any cryoprotection. As all of the cells were ruptured after thawing, we used a modified cloning method and examined nuclei from several organs for use in nuclear transfer attempts. Using brain nuclei as nuclear donors, we established embryonic stem cell lines from the cloned embryos. Healthy cloned mice were then produced from these nuclear transferred embryonic stem cells by serial nuclear transfer. Thus, nuclear transfer techniques could be used to "resurrect" animals or maintain valuable genomic stocks from tissues frozen for prolonged periods without any cryopreservation.
机译：通过核转移克隆动物提供了保存濒危哺乳动物物种的机会。然而，已经提出，由于没有活细胞可用，冷冻的灭绝物种（例如羊毛猛ma象）的“复活”是不切实际的，并且残留的基因组物质不可避免地被降解。在这里，我们报道了从在-20℃下冷冻长达16年而没有任何冷冻保护的尸体生产的克隆小鼠。由于所有细胞在融化后都会破裂，因此我们使用了一种改良的克隆方法，并检查了来自多个器官的核以用于核移植尝试。使用脑核作为核供体，我们从克隆的胚胎中建立了胚胎干细胞系。然后，通过系列核移植，从这些核移植的胚胎干细胞中生产出健康的克隆小鼠。因此，核移植技术可用于“复活”动物或从长期冷冻的组织中保持有价值的基因组储备，而无需任何冷冻保存。

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