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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Selective Saturation Of Slow Endocytosis At A Giant Glutamatergic Central Synapse Lacking Dynamin 1
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Selective Saturation Of Slow Endocytosis At A Giant Glutamatergic Central Synapse Lacking Dynamin 1

机译：缺乏动力的巨型谷氨酸能中央突触的慢吞噬作用的选择性饱和。

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摘要

Exocytosis of synaptic vesicles is rapidly followed by compensatory plasma membrane endocytosis. The efficiency of endocytosis varies with experimental conditions, but the molecular basis for this control remains poorly understood. Here, the function of dynamin 1, the neuron-specific member of a family of GTPases implicated in vesicle fission, was investigated with high temporal resolution via membrane capacitance measurements at the calyx of Held, a giant glutamatergic synapse. Endocytosis at dynamin 1 KO calyces was the same as in wild type after weak stimuli, consistent with the nearly normal ultrastructure of mutant synapses. However, following stronger stimuli, the speed of slow endocytosis, but not of other forms of endocytosis, failed to scale with the increased endocytic load. Thus, high level expression of dynamin 1 is essential to allow the slow, clathrin-mediated endocytosis, which accounts for the bulk of the endocytic response, to operate efficiently over a wide range of activity.
机译：突触小泡的胞吐作用迅速发生，随后是代偿性质膜内吞作用。内吞作用的效率随实验条件而变化，但对该控制的分子基础仍知之甚少。在这里，通过在巨大的谷氨酸能突触Held的花萼上通过膜电容测量，以高时间分辨率研究了dynamin 1的功能，dynamin 1是与囊泡裂变有关的GTP酶家族的神经元特异性成员。在弱刺激后，动力蛋白1 KO Calycaes的胞吞作用与野生型相同，这与突变突触的近乎正常的超微结构一致。然而，在较强的刺激之后，缓慢的内吞作用速度，而不是其他形式的内吞作用速度，并未随着内吞负荷的增加而缩放。因此，高表达的动力蛋白1对于使缓慢的网格蛋白介导的内吞作用（在大部分内吞反应中起作用）在广泛的活性范围内有效运行至关重要。

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