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Molecular Organization Of Gram-negative Peptidoglycan

机译:革兰氏阴性肽聚糖的分子组织

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摘要

The stress-bearing component of the bacterial cell wall-a multi-gigadalton bag-like molecule called the sacculus-is synthesized from peptidoglycan. Whereas the chemical composition and the 3-dimensional structure of the peptidoglycan subunit (in at least one conformation) are known, the architecture of the assembled sacculus is not. Four decades' worth of biochemical and electron microscopy experiments have resulted in two leading 3-D peptidoglycan models: "Layered" and "Scaffold", in which the glycan strands are parallel and perpendicular to the cell surface, respectively. Here we resolved the basic architecture of purified, frozen-hydrated sacculi through electron cryotomography. In the Gram-negative sacculus, a single layer of glycans lie parallel to the cell surface, roughly perpendicular to the long axis of the cell, encircling the cell in a disorganized hoop-like fashion.
机译:细菌细胞壁的承受压力的成分-一种多囊袋状分子(称为囊)-是由肽聚糖合成的。尽管肽聚糖亚基的化学组成和三维结构(至少一种构象)是已知的,但组装的眼球的结构却不知道。经过四十年的生化和电子显微镜实验,得出了两个领先的3-D肽聚糖模型:“分层”和“脚手架”,其中聚糖链分别平行于和垂直于细胞表面。在这里,我们通过电子冷冻断层扫描技术解析了纯化的冷冻水合囊的基本结构。在革兰氏阴性囊中,单层聚糖平行于细胞表面,大致垂直于细胞的长轴,以无序的箍状方式环绕细胞。

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