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In This Issue

机译：在这个问题上

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Fluorescent molecules are important tools for visualizing proteins and molecular pathways and for distinguishing healthy from diseased tissue. Full-animal and clinical-scale fluorescence tomo-rngraphic imaging offers great promise for studying whole organs and disease development in vivo; however, the technique is hampered because light scatters when photons interact with biological tissue, which blurs the reconstructed image and obscures important features. To reduce the scattering effect, Mark Niedre et al. utilized the earliest-arriving, and therefore minimally scattered, photons emitted from a high-speedrnpulsed laser. This technique, called early photon tomography (EPT), showed not only the tumor but also biochemical changes in adjacent tissue, thus revealing the spread and progression of Lewis lung carcinoma in live mice. These findings were confirmed by histology and ex vivo fluorescence microscopy. The authors demonstrate that EPT can be used to generate 3D images of cancers and other diseases in living animals and suggest that the technique may be particularly useful in the liver and brain, where light scattering is more pronounced than in the lungs.
机译：荧光分子是可视化蛋白质和分子途径以及区分健康组织和患病组织的重要工具。全动物和临床规模的荧光层析成像技术为研究整个器官和体内疾病发展提供了广阔的前景。然而，由于光子在与生物组织相互作用时会发生散射，从而模糊了重建的图像并掩盖了重要特征，因此该技术受到了限制。为了减少散射效应，Mark Niedre等人。利用从高速脉冲激光器发出的最早到达的光子，因此散射程度最小。这项称为早期光子断层扫描（EPT）的技术不仅显示肿瘤，而且还显示邻近组织的生化变化，从而揭示了Lewis肺癌在活小鼠中的扩散和进展。这些发现被组织学和离体荧光显微镜证实。作者证明，EPT可用于生成活体动物的癌症和其他疾病的3D图像，并建议该技术在肝脏和大脑中可能特别有用，在肝脏和大脑中，光散射比在肺中更为明显。

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