One-step Assembly In Yeast Of 25 Overlapping Dna Fragments To Form A Complete Synthetic Mycoplasma Genitalium Genome

Daniel G. Gibson; Gwynedd A. Benders; Kevin C. Axelrod; Jayshree Zaveri; Mikkel A. Algire; Monzia Moodie; Michael G. Montague; J. Craig Venter; Hamilton O. Smith; Clyde A. Hutchison III

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One-step Assembly In Yeast Of 25 Overlapping Dna Fragments To Form A Complete Synthetic Mycoplasma Genitalium Genome

机译：在25个重叠的Dna片段的酵母中一步组装即可形成完整的合成支原体生殖器基因组

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We previously reported assembly and cloning of the synthetic Mycoplasma genitalium JCVI-1.0 genome in the yeast Saccharo-myces cerevisiae by recombination of six overlapping DNA fragments to produce a 592-kb circle. Here we extend this approach by demonstrating assembly of the synthetic genome from 25 overlapping fragments in a single step. The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments.

机译：我们先前报道了通过六个重叠DNA片段的重组产生一个592-kb的圆环，在酵母酿酒酵母中合成并克隆了生殖器支原体JCVI-1.0基因组。在这里，我们通过展示一步中25个重叠片段的合成基因组组装来扩展这种方法。酵母重组的使用大大简化了从合成和天然片段组装大DNA分子的过程。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2008年第51期|20404-20409|共6页
作者
Daniel G. Gibson; Gwynedd A. Benders; Kevin C. Axelrod; Jayshree Zaveri; Mikkel A. Algire; Monzia Moodie; Michael G. Montague; J. Craig Venter; Hamilton O. Smith; Clyde A. Hutchison III;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
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关键词
in vivo dna assembly; genome synthesis; combinatorial assembly; yeast transformation; mycoplasma genitalium; synthetic biology;

机译：体内DNA组装;基因组合成;组合组装;酵母转化;生殖器支原体;合成生物学;

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专利

1. Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome [J] . Daniel G. Gibson, Gwynedd A. Benders, Cynthia Andrews-Pfannkoch, Science . 2008,第5867期

机译：生殖道支原体基因组的完整化学合成，组装和克隆
2. Comparative study of overlapping genes in the genomes of Mycoplasma genitalium and Mycoplasma pneumoniae. [J] . Fukuda Y, Washio T, Tomita M Nucleic Acids Research . 1999,第8期

机译：生殖支原体和肺炎支原体基因组中重叠基因的比较研究。
3. Comparative study of overlapping genes in the genomes of Mycoplasma genitalium and Mycoplasma pneumoniae [J] . Masaru Tomita, Takanori Washio, Yoko Fukuda Nucleic acids research . 1999,第8期

机译：生殖支原体和肺炎支原体基因组重叠基因的比较研究
4. A position-slots model for nucleosome assembly in the yeast genome based on integrated multi-platform positioning datasets [C] . Jihua Feng, Xianhua Dai, Qian Xiang, International Conference on Bio-Inspired Computing . 2009

机译：基于集成多平台定位数据集的酵母基因组中核心组装的位置槽模型
5. Yeast Genome Engineering and Synthetic Chromosome Consolidation - Building the ‘Complete’ Sc2.0 Strain with the Designer Eukaryote Genome [D] . Zhao, Yu. 2020

机译：酵母基因组工程与合成染色体固结 - 用设计师真核生物基因组建立“完整”SC2.0应变
6. One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome [O] . Daniel G. Gibson, Gwynedd A. Benders, Kevin C. Axelrod, 2008

机译：在酵母中一步一步组装25个重叠的DNA片段以形成一个完整的合成生殖器支原体基因组
7. One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome [O] . Gibson, Daniel G., Benders, Gwynedd A., Axelrod, Kevin C., 2008

机译：在酵母中一步一步组装25个重叠的DNA片段以形成一个完整的合成生殖器支原体基因组

One-step Assembly In Yeast Of 25 Overlapping Dna Fragments To Form A Complete Synthetic Mycoplasma Genitalium Genome

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