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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Recognition And Repair Of Uv Lesions In Loopstructures Of Duplex Dna By Dash-type Cryptochrome
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Recognition And Repair Of Uv Lesions In Loopstructures Of Duplex Dna By Dash-type Cryptochrome

机译：破折号隐色识别和修复双链DNA环状结构中的紫外线病变。

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DNA photolyases and cryptochromes (cry) form a family of fla voproteinsrnthat use light energy in the blue/UV-A region for the repair of UV-induced DNA lesions or for signaling, respectively. Very recently, it was shown that members of the DASH cryptochrome subclade repair specifically cyclobutane pyrimidine dimers (CPDs) in UV-damaged single-stranded DNA. Here, we report the crystal structure of Arabidopsis cryptochrome 3 with an in-situ-repaired CPD substrate in single-stranded DNA. The structure shows a binding mode similar to that of conventional DNA photolyases. Furthermore, CPD lesions in double-stranded DNA are bound and repaired with similar efficiency as in single-stranded DNA if the CPD lesion is present in a loop structure. Together, these data reveal that DASH cryptochromes catalyze light-driven DNA repair like conventional photolyases but lack an efficient flipping mechanism for interaction with CPD lesions within duplex DNA.
机译：DNA光解酶和隐色染料（啼叫声）形成黄素蛋白家族，它们分别利用蓝色/ UV-A区域的光能修复UV诱导的DNA损伤或信号传导。最近，有研究表明，DASH隐色色素小分子的成员可以修复紫外线损坏的单链DNA中的环丁烷嘧啶二聚体（CPD）。在这里，我们报告拟南芥隐色3与单链DNA中的原位修复CPD基板的晶体结构。该结构显示出与常规DNA光解酶相似的结合模式。此外，如果CPD病变存在于环状结构中，则双链DNA中的CPD病变将以与单链DNA中相似的效率结合和修复。总之，这些数据表明，DASH隐色染料像传统的光解酶一样催化光驱动的DNA修复，但缺乏与双链DNA中CPD病变相互作用的有效翻转机制。

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