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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Multiplex Protein Assays Based On Real-time Magnetic Nanotag Sensing
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Multiplex Protein Assays Based On Real-time Magnetic Nanotag Sensing

机译：基于实时磁性纳米标签感测的多重蛋白测定

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摘要

Magnetic nanotags (MNTs) are a promising alternative to fluorescent labels in biomolecular detection assays, because minute quantities of MNTs can be detected with inexpensive giant magnetore-sistive (GMR) sensors, such as spin valve (SV) sensors. However, translating this promise into easy to use and multiplexed protein assays, which are highly sought after in molecular diagnostics such as cancer diagnosis and treatment monitoring, has been challenging. Here, we demonstrate multiplex protein detection of potential cancer markers at subpicomolar concentration levels and with a dynamic range of more than four decades. With the addition of nanotag amplification, the analytic sensitivity extends into the low fM concentration range. The multianalyte ability, sensitivity, scalability, and ease of use of the MNT-based protein assay technology make it a strong contender for versatile and portable molecular diagnostics in both research and clinical settings.
机译：磁性纳米标签（MNT）在生物分子检测测定中是荧光标记的一种有前途的替代方法，因为可以用廉价的巨型磁阻（GMR）传感器（例如旋转阀（SV）传感器）检测微量的MNT。然而，将这种希望转化为易于使用的和多重蛋白测定法是一种挑战，在分子诊断学例如癌症诊断和治疗监测中广受追捧。在这里，我们展示了在亚皮摩尔浓度水平且动态范围超过四十年的潜在癌症标志物的多重蛋白质检测。通过添加nanotag扩增，分析灵敏度可扩展至低fM浓度范围。基于MNT的蛋白质测定技术的多分析物能力，灵敏度，可扩展性和易用性使其成为研究和临床环境中多功能便携式分子诊断的有力竞争者。

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