...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification and characterization of COPla- and COPlb-type vesicle classes associated with plant and algal Golgi
【24h】

Identification and characterization of COPla- and COPlb-type vesicle classes associated with plant and algal Golgi

机译：鉴定和表征与植物和藻类高尔基体相关的COPla和COPlb型囊泡类型

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Coat protein I (COPI) vesicles arise from Golgi cisternae and mediate the recycling of proteins from the Golgi back to the endoplasmic reticulum (ER) and the transport of Golgi resident proteins between cisternae. In vitro studies have produced evidence for two distinct types of COPI vesicles, but the in vivo sites of operation of these vesicles remain to be established. We have used a combination of electron tomography and immunolabeling techniques to examine Golgi stacks and associated vesicles in the cells of the scale-producing alga Scherffelia dubia and Arabidopsis preserved by high-pressure freezing/freeze-substitution methods. Five structurally distinct types of vesicles were distinguished. In Arabidopsis, COPI and COPII vesicle coat proteins as well as vesicle cargo molecules (mannosidase I and sialyltransferase-yellow fluorescent protein) were identified by immunogold labeling. In both organisms, the COPI-type vesicles were further characterized by a combination of six structural criteria: coat architecture, coat thickness, membrane structure, cargo staining, cisternal origin, and spatial distribution. Using this multiparameter structural approach, we can distinguish two types of COPI vesicles, COPIa and COPIb. COPIa vesicles bud exclusively from cis cisternae and occupy the space between cis cisternae and ER export sites, whereas the COPIb vesicles bud exclusively from medial- and trans-Golgi cisternae and are confined to the space around these latter cisternae. We conclude that COPIa vesicle-mediated recycling to the ER occurs only from cis cisternae, that retrograde transport of Golgi resident proteins by COPIb vesicles is limited to medial and trans cisternae, and that diffusion of periGolgi vesicles is restricted.
机译：外壳蛋白I（COPI）囊泡来自高尔基池，并介导高尔基池中蛋白质的循环回内质网（ER）和高尔基驻留蛋白在池之间的转运。体外研究已经为两种不同类型的COPI囊泡提供了证据，但是这些囊泡的体内操作位点尚待确定。我们已结合使用电子断层扫描和免疫标记技术来检查通过高压冷冻/冷冻替代方法保存的规模生产藻类Scherffelia dubia和拟南芥细胞中的高尔基体堆栈和相关囊泡。区分了五种结构上不同的囊泡。在拟南芥中，通过免疫金标记鉴定了COPI和COPII囊泡外壳蛋白以及囊泡货物分子（甘露糖苷酶I和唾液酸转移酶-黄色荧光蛋白）。在这两种生物中，COPI型囊泡的特征还在于六个结构标准的组合：外壳结构，外壳厚度，膜结构，货物染色，脑池起源和空间分布。使用这种多参数结构方法，我们可以区分出两种类型的COPI囊泡：COPIa和COPIb。 COPIa囊泡仅从顺式滤池出芽，并占据顺式池和内质网出口位点之间的空间，而COPIb囊泡仅从中高尔基池和反高尔基池出芽，并局限于这些后池的周围。我们得出的结论是，COPIa囊泡介导的向ER的再循环仅发生在顺式水罐中，COPIb囊泡逆行转运高尔基驻留蛋白仅限于内侧和反式水罐，而periGolgi囊泡的扩散受到限制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号