...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Reciprocal asymmetry of SYS-1/β-catenin and POP-1/TCF controls asymmetric divisions in Caenorhabditis elegans
【24h】

Reciprocal asymmetry of SYS-1/β-catenin and POP-1/TCF controls asymmetric divisions in Caenorhabditis elegans

机译：SYS-1 /β-catenin和POP-1 / TCF的相互不对称控制秀丽隐杆线虫的不对称分裂

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

β-Catenins are conserved regulators of metazoan development that function with TCF DNA-binding proteins to activate transcription. In Caenorhabditis elegans, SYS-1/β-catenin and POP-1/TCF regulate several asymmetric divisions, including that of the somatic gonadal precursor cell (SGP). In the distal but not the proximal SGP daughter, SYS-1/β-catenin and POP-1/TCF transcriptionally activate ceh-22 to specify the distal fate. Here, we investigate the distribution of SYS-1/β-catenin and its regulation. Using a rescuing transgene, VNS::SYS-1, which fuses VENUS fluorescent protein to SYS-1, we find more VNS::SYS-1 in distal than proximal SGP daughters, a phenomenon we call "SYS-1 asymmetry." In addition, SYS-1 asymmetry is seen in many other tissues, consistent with the idea that SYS-1 regulates asymmetric divisions broadly during C. elegans development. In particular, SYS-1 is more abundant in E than MS, and SYS-1 is critical for the endodermal fate. In all cases, SYS-1 is reciprocal to POP-1 asymmetry: cells with higher SYS-1 have lower POP-1, and vice versa. SYS-1 asymmetry is controlled posttranslationally and relies on frizzled and dishevelled ho-mologs, which also control POP-1 asymmetry. Therefore, upstream regulators modulate the SYS-1 to POP-1 ratio by increasing SYS-1 and decreasing POP-1 within the same cell. By contrast, SYS-1 asymmetry does not rely on WRM-1, which appears specialized for POP-1 asymmetry. We suggest a two-pronged pathway for control of SYS-1 :POP-1, which can robustly accomplish differential gene expression in daughters of an asymmetric cell division.
机译：β-连环素是后生动物发育的保守调节物，与TCF DNA结合蛋白一起激活转录。在秀丽隐杆线虫中，SYS-1 /β-catenin和POP-1 / TCF调节几种不对称分裂，包括体细胞性腺前体细胞（SGP）的分裂。在远端但不是近端SGP子代中，SYS-1 /β-catenin和POP-1 / TCF转录激活ceh-22来确定远端命运。在这里，我们研究了SYS-1 /β-catenin的分布及其调控。使用将VENUS荧光蛋白融合到SYS-1的拯救性转基因VNS :: SYS-1，我们发现远侧SGP女儿的远端VNS :: SYS-1比我们称之为“ SYS-1不对称”的现象更多。另外，在许多其他组织中也发现了SYS-1不对称性，这与在秀丽隐杆线虫发育过程中SYS-1广泛调节不对称分裂的想法是一致的。特别是，SYS-1在E方面比MS更为丰富，而SYS-1对于内胚层的命运至关重要。在所有情况下，SYS-1都是POP-1不对称性的对立：SYS-1较高的单元格的POP-1较低，反之亦然。 SYS-1不对称性在翻译后受到控制，并且依赖于卷曲和蓬乱的同源物，后者也控制POP-1不对称性。因此，上游调节器通过增加SYS-1并减少同一单元中的POP-1来调节SYS-1与POP-1的比率。相反，SYS-1不对称不依赖于WRM-1，后者似乎专门用于POP-1不对称。我们建议控制SYS-1：POP-1的两管齐下的途径，可以在不对称细胞分裂的子代中稳健地完成差异基因的表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号