...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Interaction between transcellular and paracellular water transport pathways through Aquaporin 5 and the tight junction complex
【24h】

Interaction between transcellular and paracellular water transport pathways through Aquaporin 5 and the tight junction complex

机译：通过Aquaporin 5和紧密连接复合体的跨细胞和旁细胞水运输途径之间的相互作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To investigate potential physiological interactions between the transcellular and paracellular pathways of water transport, we asked whether targeted deletion of Aquaporin 5 (AQP5), the major transcellular water transporter in salivary acinar cells, affected paracellular transport of 4-kDa FITC-labeled dextran (FITC-D), which is transported through the paracellular but not the trans-cellular route. After i.v. injection of FITC-D into either AQP5 wild-type or AQP5~(-/-) mice and saliva collection for fixed time intervals, we show that the relative amount of FITC-D transported in the saliva of AQP5~(-/-) mice is half that in matched AQP5~(+/+) mice, indicating a 2-fold decrease in permeability of the paracellular barrier in mice lacking AQP5. We also found a significant difference in the proportion of transcellular vs. paracellular transport between male and female mice. Freeze-fracture electron microscopy revealed an increase in the number of tight junction strands of both AQP5~(+/+) and AQP5~(-/-) male mice after pilo-carpine stimulation but no change in strand number in female mice. Average acinar cell volume was increased by ≈ 1.4-fold in glands from AQP5~(-/-) mice, suggesting an alteration in the volume-sensing machinery of the cell. Western blots revealed that expression of Claudin-7, Claudin-3, and Occludin, critical proteins that regulate the permeability of the tight junction barrier, were significantly decreased in AQP5~(-/-) compared with AQP5~(+/+) salivary glands. These findings reveal the existence of a gender-influenced molecular mechanism involving AQP5 that allows transcellular and paracellular routes of water transport to act in conjunction.
机译：为了研究水运输的跨细胞和旁细胞途径之间的潜在生理相互作用，我们询问是否有针对性地缺失唾液腺细胞中主要的跨细胞水转运蛋白Aquaporin 5（AQP5）是否影响了4-kDa FITC标记的葡聚糖（FITC）的旁细胞运输。 -D），其通过细胞旁途径而非跨细胞途径转运。在i.v.之后将FITC-D注入AQP5野生型或AQP5〜（-/-）小鼠并按固定时间间隔收集唾液，我们显示FITC-D在AQP5〜（-/-）唾液中运输的相对量小鼠是匹配的AQP5〜（+ / +）小鼠的一半，表明缺乏AQP5的小鼠的副细胞屏障通透性降低了2倍。我们还发现雄性和雌性小鼠的跨细胞运输与旁细胞运输的比例存在显着差异。冷冻断裂电镜观察到，毛果芸香碱刺激后，AQP5〜（+ / +）和AQP5〜（-/-）雄性小鼠的紧密连接链数目增加，但雌性小鼠的链数目没有变化。在AQP5〜（-/-）小鼠的腺体中，平均腺泡细胞体积增加了约1.4倍，表明细胞的体积感应机制发生了变化。 Western印迹表明，与唾液中的AQP5〜（+ / +）相比，调节紧密连接屏障通透性的关键蛋白Claudin-7，Claudin-3和Occludin的表达明显降低。腺体。这些发现揭示了涉及AQP5的受性别影响的分子机制的存在，该机制允许水转运的跨细胞和旁细胞途径共同起作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号