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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Heterotrimeric G protein signaling in the Arabidopsis unfolded protein response
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Heterotrimeric G protein signaling in the Arabidopsis unfolded protein response

机译：拟南芥中的异源三聚体G蛋白信号传导展开的蛋白反应

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摘要

We present evidence that heterotrimeric G protein signaling is involved in cell death associated with the unfolded protein response (UPR) in Arabidopsis. Seedlings of homozygous agb1-2 (Gβ-null mutation) mutant plants are markedly more resistant to growth inhibition by the protein glycosylation inhibitor tunicamy-cin (Tm) than either wild-type plants or gpa1-4 (Gα-null mutation) mutants. Leaves of older Gβ mutant plants show much less cell death when infiltrated with Tm than leaves of wild-type plants. The transcriptional response of Gβ mutant plants to Tm is less pronounced than that of wild-type plants, as is the accumulation of BiP chaperone proteins. A majority of the Arabidopsis Gβ protein is associated with the endoplasmic reticulum (ER) and cofractionates with membrane-associated ER luminal BiP. Consistent with its ER localization, Gβ protein is degraded during the UPR, whereas Gα protein is not. Taken together, these observations imply that the Gβ protein, which forms a stable heterodimer with the Gγ subunit, is involved in the signaling events that trigger UPR-associated cell death. The different Tm sensitivities of Gα and Gβ mutants, the ER localization of Gβ, and the differential stabilities of Gα and Gβ proteins during the UPR suggest that the Gβγ complex serves a signaling function in the ER independent of its function in the Gαβγ heterotrimer.
机译：我们目前的证据，异源三聚体G蛋白信号参与拟南芥中未折叠的蛋白应答（UPR）相关的细胞死亡。纯合agb1-2（Gβ-无效突变）突变体植物的幼苗比野生型植物或gpa1-4（Gα-无效突变）突变体对蛋白糖基化抑制剂衣霉素（Tm）的生长抑制具有明显的抵抗力。与野生型植物的叶子相比，老的Gβ突变植物的叶子在被Tm浸润后显示出更少的细胞死亡。 Gβ突变体植物对Tm的转录反应不如野生型植物明显，BiP伴侣蛋白的积累也是如此。大部分拟南芥Gβ蛋白与内质网（ER）相关，并与膜相关的ER腔BiP共分馏。与其ER定位相一致，UPR期间Gβ蛋白被降解，而Gα蛋白则不被降解。综上所述，这些观察结果暗示与Gγ亚基形成稳定异二聚体的Gβ蛋白参与触发UPR相关细胞死亡的信号转导事件。在UPR期间，Gα和Gβ突变体的不同Tm敏感性，Gβ的ER定位以及Gα和Gβ蛋白质的不同稳定性表明，Gβγ复合物在ER中起信号传递功能，而与它在Gαβγ异三聚体中的功能无关。

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