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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The proton pumping pathway of bovine heart cytochrome c oxidase
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The proton pumping pathway of bovine heart cytochrome c oxidase

机译：牛心脏细胞色素c氧化酶的质子泵送途径

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摘要

X-ray structures of bovine heart cytochrome c oxidase have suggested that the enzyme, which reduces O_2 in a process coupled with a proton pumping process, contains a proton pumping pathway (H-pathway) composed of a hydrogen bond network and a water channel located in tandem across the enzyme. The hydrogen bond network includes the peptide bond between Tyr-440 and Ser-441, which could facilitate unidirectional proton transfer. Replacement of a possible proton-ejecting aspartate (Asp-51) at one end of the H-pathway with asparagine, using a stable bovine gene expression system, abolishes the proton pumping activity without influencing the O_2 reduction function. Blockage of either the water channel by a double mutation (Val386Leu and Met390Trp) or proton transfer through the peptide by a Ser441Pro mutation was found to abolish the proton pumping activity without impairment of the O_2 reduction activity. These results significantly strengthen the proposal that H-pathway is involved in proton pumping.
机译：牛心脏细胞色素C氧化酶的X射线结构表明，该酶在与质子泵送过程相结合的过程中还原O_2，其包含由氢键网络和位于水通道中的质子泵送通道（H通道）跨酶串联。氢键网络包括Tyr-440和Ser-441之间的肽键，可以促进单向质子转移。使用稳定的牛基因表达系统，用天冬酰胺取代H通路一端可能的质子喷射天冬氨酸（Asp-51），从而消除了质子泵送活性，而不会影响O_2还原功能。发现通过双重突变（Val386Leu和Met390Trp）阻塞水通道或通过Ser441Pro突变通过肽的质子传递被阻断，从而消除了质子泵送活性而不损害O_2还原活性。这些结果显着增强了H通路参与质子泵送的提议。

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