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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Embryonic chimerism does not induce tolerance in an invertebrate model organism
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Embryonic chimerism does not induce tolerance in an invertebrate model organism

机译：胚胎嵌合体不会在无脊椎动物模型生物中诱导耐受

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摘要

In colonial marine invertebrates, allorecognition restricts somatic fusion and thus, chimerism, to histocompatible individuals. Little is understood, however, about how invertebrates respond to chimerism formed across histocompatibility barriers or whether embryonic exposure to histoincompatible cells induces allotolerance. We here evaded natural allorecognition barriers by generating well mixed embryonic chimeras of Hydractinia symbiolongicarpus (Cnidaria:Hydrozoa) and developed molecular markers to detect chimerism in both histocompatible and histoincompatible settings. Histocompatible chimeras exhibited markedly higher growth rates and survivorship than histoincompatible pairings. Histoincompatible chimeras were unstable, with chimerism being undetectable by 4 wk of age. In contrast, colonies generated from histocompatible pairings remained chimeric at markedly higher frequencies and longer durations. Histoincompatible chimeras that lost detectable chimerism retained the fusibility/rejection characteristics of the remaining component of the chimera but not that of the lost component. Chimerism across histocompatibility barriers in an invertebrate model organism was unstable and did not induce tolerance.
机译：在殖民地的海洋无脊椎动物中，同位基因识别将体细胞融合以及嵌合体限制于组织相容的个体。然而，关于无脊椎动物如何对跨组织相容性壁垒形成的嵌合反应或胚胎暴露于组织不相容细胞是否诱发同种异体耐受性了解甚少。我们在这里通过产生混合性强的Hydractinia symbiolongicarpus（Cnidaria：Hydrozoa）的胚胎嵌合体来逃避自然的同种异体认知障碍，并开发了分子标记物来检测在组织相容性和组织相容性环境中的嵌合体。组织相容性嵌合体比组织相容性配对表现出明显更高的生长速率和存活率。组织相容性嵌合体是不稳定的，在4周龄之前无法发现嵌合体。相反，由组织相容性配对产生的菌落仍以明显更高的频率和更长的持续时间嵌合。失去可检测的嵌合现象的组织相容性嵌合体保留了嵌合体其余成分的融合性/排斥性，但没有丢失成分的融合性/排斥性。在无脊椎动物模型生物中，跨组织相容性障碍的嵌合是不稳定的，不会诱导耐受。

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