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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A role for noncoding transcription in activation of the yeast PHO5 gene
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A role for noncoding transcription in activation of the yeast PHO5 gene

机译：非编码转录在酵母PHO5基因激活中的作用

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摘要

Noncoding, or intergenic, transcription by RNA polymerase Ⅱ (RNA-PⅡ) is remarkably widespread in eukaryotic organisms, but the effects of such transcription remain poorly understood. Here we show that noncoding transcription plays a role in activation, but not repression, of the Saccharomyces cerevisiae PHO5 gene. Histone eviction from the PHOS promoter during activation occurs with normal kinetics even in the absence of the PHO5 TATA box, showing that transcription of the gene itself is not required for promoter remodeling. Nevertheless, we find that mutations that impair transcript elongation by RNAPⅡ affect the kinetics of histone eviction from the PHO5 promoter. Most dramatically, inactivation of RNAPⅡ itself abolishes eviction completely. Under repressing conditions, an ≈ 2.4-kb noncoding exosome-degraded transcript is detected that originates near the PHOS termination site and is transcribed in the antisense direction. Abrogation of this transcript delays chromatin remodeling and subsequent RNAPⅡ recruitment to PHOS upon activation. We propose that noncoding transcription through positioned nucleosomes can enhance chromatin plasticity so that chromatin remodeling and activation of traversed genes occur in a timely manner.
机译：RNA聚合酶Ⅱ（RNA-PⅡ）进行的非编码或基因间转录在真核生物中非常普遍，但对这种转录的作用仍知之甚少。在这里，我们显示非编码转录在酿酒酵母PHO5基因的激活而不是抑制中起作用。即使在没有PHO5 TATA盒的情况下，在激活过程中从PHOS启动子中清除组蛋白也能以正常动力学进行，这表明启动子重塑不需要基因本身的转录。然而，我们发现损害RNAPⅡ转录本延伸的突变会影响从PHO5启动子中逐出组蛋白的动力学。最显着的是，RNAPⅡ本身的失活完全消除了逐出。在阻抑条件下，检测到≈2.4-kb非编码外来体降解的转录本，其起源于PHOS终止位点附近，并沿反义方向转录。该转录本的废除延迟了染色质重塑和随后的RNAPⅡ在活化后募集到PHOS。我们建议通过定位核小体的非编码转录可以增强染色质的可塑性，从而使染色质重塑和遍历基因的激活及时发生。

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