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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Controlling hydrogelation kinetics by peptide design for three-dimensional encapsulation and injectable delivery of cells
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Controlling hydrogelation kinetics by peptide design for three-dimensional encapsulation and injectable delivery of cells

机译：通过肽设计控制水凝胶动力学，用于三维封装和细胞注射

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摘要

A peptide-based hydrogelation strategy has been developed that allows homogenous encapsulation and subsequent delivery of C3H10t1/2 mesenchymal stem cells. Structure-based peptide design afforded MAX8, a 20-residue peptide that folds and self-assembles in response to DMEM resulting in mechanically rigid hydrogels. The folding and self-assembly kinetics of MAX8 have been tuned so that when hydrogelation is triggered in the presence of cells, the cells become homogeneously impregnated within the gel. A unique characteristic of these gel-cell constructs is that when an appropriate shear stress is applied, the hydrogel will shear-thin resulting in a low-viscosity gel. However, after the application of shear has stopped, the gel quickly resets and recovers its initial mechanical rigidity in a near quantitative fashion. This property allows gel/cell constructs to be delivered via syringe with precision to target sites. Homogenous cellular distribution and cell viability are unaffected by the shear thinning process and gel/cell constructs stay fixed at the point of introduction, suggesting that these gels may be useful for the delivery of cells to target biological sites in tissue regeneration efforts.
机译：已经开发了一种基于肽的水凝胶化策略，该策略允许同质封装并随后递送C3H10t1 / 2间充质干细胞。基于结构的肽设计提供了MAX8，这是一种20残基的肽，可对DMEM进行折叠和自组装，从而产生机械刚性的水凝胶。已对MAX8的折叠和自组装动力学进行了调整，以便在存在细胞的情况下触发水凝胶化时，细胞会均匀地浸入凝胶中。这些凝胶细胞构建体的独特特征是，当施加适当的剪切应力时，水凝胶会剪切稀薄，从而形成低粘度的凝胶。但是，在停止施加剪切力之后，凝胶迅速复位并以接近定量的方式恢复了其初始机械刚度。该性质允许凝胶/细胞构建体通过注射器精确地递送至靶位。均质的细胞分布和细胞活力不受剪切稀化过程的影响，并且凝胶/细胞构建体在引入时保持固定，表明这些凝胶可用于组织再生工作中将细胞递送至靶标生物部位。

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