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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Conserved domain structure of pentatricopeptide repeat proteins involved in chloroplast RNA editing
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Conserved domain structure of pentatricopeptide repeat proteins involved in chloroplast RNA editing

机译：参与叶绿体RNA编辑的五肽重复序列蛋白的保守结构域结构

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摘要

The pentatricopeptide repeat (PPR) proteins form one of the largest families in higher plants and are believed to be involved in the posttranscriptional processes of gene expression in plant organelles. It has been shown by using a genetic approach focusing on NAD(P)H dehydrogenase (NDH) activity that a PPR protein CRR4 is essential for a specific RNA editing event in chloroplasts. Here, we discovered Arabidopsis crr21 mutants that are specifically impaired in the RNA editing of the site 2 of ndhD (ndhD-2), which encodes a subunit of the NDH complex. The CRR21 gene encodes a member of the PPR protein family. The RNA editing of ndhD-2 converts the Ser-128 of NdhD to leucine. In crr21, the activity of the NDH complex is specifically impaired, suggesting that the Ser128Leu change has important consequences for the function of the NDH complex. Both CRR21 and CRR4 belong to the E+ subgroup in the PLS subfamily that is characterized by the presence of a conserved C-terminal region (the E/E+ domain). This E/E+ domain is highly conserved and exchangeable between CRR21 and CRR4, although it is not essential for the RNA binding. Our results suggest that the E/E+ domain has a common function in RNA editing rather than of recognizing specific RNA sequences.
机译：五肽重复序列（PPR）蛋白是高等植物中最大的家族之一，被认为与植物细胞器中基因表达的转录后过程有关。通过使用专注于NAD（P）H脱氢酶（NDH）活性的遗传方法已显示，PPR蛋白CRR4对于叶绿体中的特定RNA编辑事件至关重要。在这里，我们发现了拟南芥crr21突变体，该突变体在编码ndhD（ndhD-2）的位点2的RNA编辑中特别受损，该位点编码NDH复合物的一个亚基。 CRR21基因编码PPR蛋白家族的成员。 ndhD-2的RNA编辑将NdhD的Ser-128转换为亮氨酸。在crr21中，NDH复合物的活性特别受损，这表明Ser128Leu的变化对NDH复合物的功能具有重要影响。 CRR21和CRR4都属于PLS亚家族中的E +子组，其特征是存在保守的C端区域（E / E +域）。该E / E +域在CRR21和CRR4之间是高度保守的和可交换的，尽管它对于RNA结合不是必需的。我们的结果表明，E / E +域在RNA编辑中具有共同的功能，而不是识别特定的RNA序列。

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