...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Population genetic structure of Plasmodium falciparum in the two main African vectors, Anopheles gambiae and Anopheles funestus
【24h】

Population genetic structure of Plasmodium falciparum in the two main African vectors, Anopheles gambiae and Anopheles funestus

机译：非洲两个主要媒介冈比亚按蚊和真菌按蚊中恶性疟原虫的种群遗传结构

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We investigated patterns of genetic diversity of Plasmodium falciparum associated with its two main African vectors: Anopheles gambiae and Anopheles funestus. We dissected 10,296 wild-caught mosquitoes from three tropical sites, two in Cameroon (Simbock and Tibati, separated by 320 km) and one in Kenya (Rota, > 2,000 km from the other two sites). We assayed seven microsatellite loci in 746 oocysts from 183 infected mosquito guts. Genetic polymorphism was very high in parasites isolated from both vector species. The expected heterozygosity (H_E) was 0.79 in both species; the observed heterozygosities (H_O) were 0.32 in A. funestus and 0.42 in A. gambiae, indicating considerable inbreeding within both vector species. Mean selfing (s) between genetically identical gametes was s = 0.33. Differences in the rate of inbreeding were statistically insignificant among sites and between the two vector species. As expected, because of the high rate of inbreeding, linkage disequilibrium was very high; it was significant for all 21 loci pairs in A. gambiae and for 15 of 21 pairs in A. funestus, although only two pairwise comparisons were between loci on the same chromosome. Overall, the genetic population structure of P. falciparum, as evaluated by F statistics, was predominantly clonal rather than panmictic, a population structure that facilitates the spread of antimalarial drug and vaccine resistance and thus may impair the effectiveness of malaria control efforts.
机译：我们调查了恶性疟原虫与其两个主要的非洲载体：冈比亚按蚊和真菌按蚊的遗传多样性模式。我们解剖了来自三个热带地区的10,296头野生蚊子，其中两个在喀麦隆（辛博克和蒂巴蒂，相距320公里），另一个在肯尼亚（罗塔（距另外两个地方> 2000公里））。我们在183个被感染的蚊子肠道中的746个卵囊中检测了七个微卫星基因座。从这两种载体物种中分离出的寄生虫的遗传多态性很高。两种物种的预期杂合度（H_E）均为0.79；在真菌中观察到的杂合度（H_O）为0.32，在冈比亚按蚊中观察到的杂合度（H_O）为0.42，表明这两个载体物种都存在大量近交。基因相同配子之间的平均自交为s = 0.33。在位点之间以及两个载体物种之间的近交率差异在统计学上不显着。不出所料，由于近交率很高，连锁不平衡非常高。尽管在同一条染色体上的基因座之间只有两个成对比较，但对于冈比亚拟南芥中的所有21个基因座对和真菌曲霉中的21个对中的15个都具有显着意义。总体而言，通过F统计评估，恶性疟原虫的遗传种群结构主要是克隆的而不是Panmictic的，这种种群结构促进了抗疟疾药物和疫苗耐药性的传播，因此可能削弱疟疾控制工作的有效性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号