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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Functional redundancy of worm spliceosomal proteins U1A and U2B″
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Functional redundancy of worm spliceosomal proteins U1A and U2B″

机译：蠕虫剪接蛋白U1A和U2B的功能冗余”

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摘要

In Caenorhabditis elegans, the small nuclear ribonucleoprotein (snRNP)-associated proteins U1A and U2B″ are ≈50% identical to each other, and neither bears signature characteristics of mammalian U1A or U2B″ or the single Drosophila homolog, SNF. We show here that the genes that encode these proteins (rnp-2 and rnp-3) are cotranscribed in an operon, and that ribonucleoprotein RNP-2 is U1 snRNP-associated (U1A) whereas RNP-3 is U2 snRNP-associated (U2B″). U2B″ interacts with U2 even in the absence of another U2 snRNP protein, U2A′. Like U1A and U2B″ from yeast, plants, and vertebrates, worm U1A and U2B″ are more similar to each other than they are to other U1A or U2B″ proteins, respectively. Even though U1A and U2B″ interact with different snRNPs, they are functionally redundant; knockout of both is required for a lethal phenotype. Interestingly, U1A associates with U2 RNA when U2B″ is deleted. Thus, the two members of this gene family normally function as components of different snRNPs but apparently remain capable of performing the function of the other. Redundancy results from the fact that one protein can substitute for the other, even though it normally does not.
机译：在秀丽隐杆线虫中，与小核糖核糖核蛋白（snRNP）相关的蛋白U1A和U2B''彼此≈50％相同，并且都不具有哺乳动物U1A或U2B''的特征或单个果蝇同系物SNF。我们在这里显示，编码这些蛋白质（rnp-2和rnp-3）的基因在操纵子中共转录，而核糖核蛋白RNP-2与U1 snRNP相关（U1A），而RNP-3与U2 snRNP相关（U2B） ”）。即使在没有另一个U2 snRNP蛋白U2A'的情况下，U2B''也能与U2相互作用。就像来自酵母，植物和脊椎动物的U1A和U2B''一样，蠕虫U1A和U2B''彼此之间的相似性比与其他U1A或U2B''蛋白的相似。即使U1A和U2B''与不同的snRNP交互，它们在功能上是冗余的；致死表型都需要敲除两者。有趣的是，当U2B''被删除时，U1A与U2 RNA缔合。因此，该基因家族的两个成员通常作为不同的snRNPs的组件起作用，但显然仍然能够执行另一个功能。冗余是由于一种蛋白质可以替代另一种蛋白质，尽管通常不能替代。

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