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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The two-pore channel TPK1 gene encodes the vacuolar K~+ conductance and plays a role in K~+ homeostasis
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The two-pore channel TPK1 gene encodes the vacuolar K~+ conductance and plays a role in K~+ homeostasis

机译：两孔通道TPK1基因编码液泡K〜+电导并在K〜+稳态中起作用

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摘要

The Arabidopsis thaliana genome contains five genes that encode two pore K~+ (TPK) channels. The most abundantly expressed isoform of this family, TPK1, is expressed at the tonoplast where it mediates K~+-selective currents between cytoplasmic and vacuolar compartments. TPK1 open probability depends on both cytoplasmic Ca~(2+) and cytoplasmic pH but not on the tonoplast membrane voltage. The channel shows intrinsic rectification and can be blocked by Ba~(2+), tetraethylammonium, and quinine. TPK1 current was found in all shoot cell types and shows all of the hallmarks of the previously described vacuolar K (VK) tonoplast channel characterized in guard cells. Characterization of TPK1 loss-of-function mutants and TPK1-overexpressing plants shows that TPK1 has a role in intracellular K~+ homeostasis affecting seedling growth at high and low ambient K~+ levels. In stomata, TPK1 function is consistent with vacuolar K~+ release, and removal of this channel leads to slower stomatal closure kinetics. During germination, TPK1 contributes to the radicle development through vacuolar K~+ deposition to provide expansion growth or in the redistribution of essential minerals.
机译：拟南芥基因组包含五个编码两个孔K +（TPK）通道的基因。该家族中表达最丰富的同工型TPK1在液泡膜中表达，在液泡膜中它介导细胞质和液泡区室之间的K〜+选择电流。 TPK1开放的可能性取决于细胞质Ca〜（2+）和细胞质pH，而不取决于液泡膜膜电压。该通道显示出固有的整流作用，并可能被Ba〜（2+），四乙铵和奎宁所阻断。在所有芽细胞类型中均发现了TPK1电流，并显示了先前描述的以保卫细胞为特征的液泡K（VK）液泡膜通道的所有特征。对TPK1功能丧失突变体和TPK1过表达植物的表征表明，TPK1在高和低环境K〜+水平下均影响细胞内K〜+稳态，影响幼苗的生长。在气孔中，TPK1的功能与液泡中的K +释放相一致，并且该通道的去除导致气孔关闭动力学变慢。在发芽过程中，TPK1通过液泡K +沉积促进胚根发育，从而提供膨胀生长或必需矿物质的重新分布。

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