...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Canalization of segmentation and its evolution in Drosophila
【24h】

Canalization of segmentation and its evolution in Drosophila

机译：果蝇的节段化及其进化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Segmentation in Drosophila embryogenesis occurs through a hierarchical cascade of regulatory gene expression driven by the establishment of a diffusion-mediated morphogen gradient. Here, we investigate the response of this pattern formation process to genetic variation and evolution in egg size. Specifically, we ask whether spatial localization of gap genes Kruppel (Kr) and giant (gt) and the pair-rule gene even-skipped (eve) during cellulariza-tion is robust to genetic variation in embryo length in three Drosophila melanogaster isolines and two closely related species. We identified two wild-derived strains of D. melanogaster whose eggs differ by ≈25% in length when reared under identical conditions. These two lines, a D. melanogaster laboratory stock (w1118), and offspring from crosses between the lines all exhibit precise scaling in the placement of gap and pair-rule gene expression along the anterior-posterior axis in relation to embryo length. Genetic analysis indicates that this scaling is maternally controlled. Maternal regulation of scaling must be required for consistent localization of segmentation gene expression because embryo size, a genetically variable and adaptive trait, is maternally inherited. We also investigated spatial scaling between these D. melanogaster lines and single lines of Drosophila sechellia and Drosophila simulans, the latter two differing by ≈25% in egg length. In contrast to the robust scaling we observed within species, localization of gene expression relative to embryo length differs significantly between the three species. Thus, the developmental mechanism that assures robust scaling within a species does not prevent rapid evolution between species.
机译：果蝇胚胎发生中的分段是通过建立由扩散介导的形态发生子梯度驱动的调控基因表达的分级级联发生的。在这里，我们调查这种模式形成过程对卵形的遗传变异和进化的响应。具体来说，我们询问在细胞果蝇化过程中，间隙基因Kruppel（Kr）和巨人（gt）以及成对规则基因（eve）的空间定位是否对三种果蝇同种异型和两种果蝇的胚胎长度遗传变异具有鲁棒性密切相关的物种。我们鉴定了两个野生D. melanogaster菌株，它们在相同条件下饲养时卵长相差约25％。这两条线，即黑腹果蝇实验室种（w1118），以及两线之间的杂交后代，均沿前后轴相对于胚胎长度显示出精确的缩放比例，即缺口和成对规则基因表达的位置。遗传分析表明，这种缩放由母亲控制。分割基因表达的一致定位必须要求缩放比例的母体调节，因为胚胎大小是遗传上的可变性和适应性特征，是母体遗传的。我们还研究了这些D. melanogaster系与果蝇sechellia和Drosophila simulans的单系之间的空间比例，后两者在卵长方面相差约25％。与我们在物种内观察到的稳健缩放相反，在三种物种之间，基因表达相对于胚胎长度的定位存在显着差异。因此，确保物种内稳健缩放的发育机制不会阻止物种之间的快速进化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号