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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Interaction of a plant virus-encoded protein with the major nucleolar protein fibrillarin is required for systemic virus infection
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Interaction of a plant virus-encoded protein with the major nucleolar protein fibrillarin is required for systemic virus infection

机译：系统病毒感染需要植物病毒编码蛋白与主要核仁蛋白原纤维蛋白的相互作用

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摘要

The nucleolus and specific nucleolar proteins are involved in the life cycles of some plant and animal viruses, but the functions of these proteins and of nucleolar trafficking in virus infections are largely unknown. The ORF3 protein of the plant virus, groundnut rosette virus (an umbravirus), has been shown to cycle through the nucleus, passing through Cajal bodies to the nucleolus and then exiting back into the cytoplasm. This journey is absolutely required for the formation of viral ribonucleoprotein particles (RNPs) that, themselves, are essential for the spread of the virus to noninocu-lated leaves of the shoot tip. Here, we show that these processes rely on the interaction of the ORF3 protein with fibrillarin, a major nucleolar protein. Silencing of the fibrillarin gene prevents longdistance movement of groundnut rosette virus but does not affect viral replication or cell-to-cell movement. Repressing fibrillarin production also localizes the ORF3 protein to multiple Cajal body-like aggregates that fail to fuse with the nucleolus. Umbraviral ORF3 protein and fibrillarin interact in vitro and, when mixed with umbravirus RNA, form an RNP complex. This complex has a filamentous structure with some regular helical features, resembling the RNP complex formed in vivo during umbravirus infection. The filaments formed in vitro are infectious when inoculated to plants, and their infectivity is resistant to RNase. These results demonstrate previously undescribed functions for fibrillarin as an essential component of translocatable viral RNPs and may have implications for other plant and animal viruses that interact with the nucleolus.
机译：核仁蛋白和特定的核仁蛋白参与某些动植物病毒的生命周期，但是这些蛋白的功能以及核仁在病毒感染中的运输功能尚不清楚。已显示植物病毒花生玫瑰花结病毒（一种伞形病毒）的ORF3蛋白在核中循环，通过Cajal体到达核仁，然后返回细胞质。病毒核糖核蛋白颗粒（RNP）的形成是绝对必要的，而病毒核糖核蛋白颗粒（RNPs）本身对病毒传播到芽尖端的非无菌叶至关重要。在这里，我们表明这些过程依赖于ORF3蛋白与纤维蛋白（一种主要的核仁蛋白）的相互作用。沉默原纤维蛋白基因可防止花生玫瑰花结病毒长距离运动，但不影响病毒复制或细胞间运动。抑制原纤维蛋白的产生还将ORF3蛋白定位于无法与核仁融合的多个Cajal体样聚集体。伞病毒ORF3蛋白和纤维蛋白在体外相互作用，当与伞病毒RNA混合时，会形成RNP复合体。该复合物具有丝状结构，具有一些规则的螺旋特征，类似于在本影象病毒感染期间在体内形成的RNP复合物。体外形成的细丝在接种到植物中时具有传染性，其感染性对RNase具有抗性。这些结果证明了原纤维蛋白的先前未曾描述的功能，它是可转移病毒RNP的基本组成部分，可能对与核仁相互作用的其他动植物病毒有影响。

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