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A GeneTrek analysis of the maize genome

机译:玉米基因组的GeneTrek分析

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摘要

Analysis of the sequences of 74 randomly selected BACs demonstrated that the maize nuclear genome contains ≈ 37,000 candidate genes with homologues in other plant species. An additional ≈ 5,500 predicted genes are severely truncated and probably pseudogenes. rnThe distribution of genes is uneven, with ≈ 30% of BACs containing no genes. BAC gene density varies from 0 to 7.9 per 100 kb, whereas most gene islands contain only one gene. The average number of genes per gene island is 1.7. Only 72% of these genes show collinearity with the rice genome. Particular LTR retrotransposon rnfamilies (e.g., Gyma) are enriched on gene-free BACs, most of which do not come from pericentromeres or other large heterochromatic rnregions. Gene-containing BACs are relatively enriched in different families of LTR retrotransposons (e.g., Ji). Two major bursts of LTR retrotransposon activity in the last 2 million years are responsible for the large size of the maize genome, but only the more recent of these is well represented in gene-containing BACs, suggesting that LTR retrotransposons are more efficiently removed in these domains. The results demonstrate that sample sequencing and careful annotation of a few randomly selected BACs can provide a robust description of a complex plant genome.
机译:对74个随机选择的BAC序列的分析表明,玉米核基因组包含≈37,000个候选基因,与其他植物物种具有同源性。另外约5500个预测基因被严重截断,并且可能是假基因。 rn基因的分布不均匀,大约30%的BAC不包含基因。 BAC基因密度每100 kb从0到7.9不等,而大多数基因岛仅包含一个基因。每个基因岛的平均基因数目为1.7。这些基因中只有72%显示与水稻基因组共线性。特定的LTR反转录转座子家族(例如Gyma)富含无基因的BAC,其中大多数都不来自着丝粒或其他较大的异色rn地区。含基因的BAC在LTR逆转座子的不同家族中相对富集(例如Ji)。在过去的200万年中,两次重大的LTR反转录转座子活性导致了玉米基因组的庞大,但只有最近的一次才在含基因的BAC中得到很好的体现,这表明LTR反转录转座子在这些基因中的去除效率更高。域。结果表明,样品测序和一些随机选择的BAC的仔细注解可以提供复杂植物基因组的可靠描述。

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