...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >In situ dissection of a Polycomb response element in Drosophila melanogaster
【24h】

In situ dissection of a Polycomb response element in Drosophila melanogaster

机译：果蝇中Polycomb反应元件的原位解剖

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Genes of the Polycomb group maintain long-term, segment-specific repression of the homeotic genes in Drosophila. DNA targets of Polycomb group proteins, called Polycomb response elements (PREs), have been defined by several assays, but they have not been dissected in their original chromosomal context. An enhanced method of gene conversion was developed to generate a series of small, targeted deletions encompassing the best-studied PRE, upstream of the Ultrabithorax (Ubx) transcription unit in the bithorax complex. Deletions that removed an essential 185-bp core of the PRE caused anterior misexpression of Ubx and posterior segmental transformations, including the conversion of the third thoracic segment toward a duplicate first abdominal segment. These phenotypes were variable, suggesting some cooperation between this PRE and others in the bithorax complex. Larger deletions up to 3 kb were also created, which removed DNA sites reportedly needed for Ubx activation, including putative trithorax response elements. These deletions resulted in neither loss of Ubx expression nor loss-of-function phenotypes. Thus, the 3-kb region including the PRE is required for repression, but not for activation, of Ubx.
机译：Polycomb组的基因对果蝇的同源基因具有长期的，片段特异性的抑制作用。 Polycomb组蛋白的DNA靶标称为Polycomb响应元件（PRE），已通过多种测定法进行了定义，但尚未在其原始染色体背景下进行解剖。开发了一种增强的基因转换方法，以产生一系列小的，针对性的缺失，其中包括研究最好的PRE，该序列位于bithorax复合体中Ultrabithorax（Ubx）转录单元的上游。删除删除了PRE的一个必不可少的185 bp核心，导致Ubx的前位错表达和后节段转换，包括将第三胸段转换为重复的第一腹段。这些表型是可变的，表明该PRE和其他在位胸复合物中的合作。还创建了最大3 kb的较大缺失，据报道删除了Ubx激活所需的DNA位点，包括推定的三胸反应元件。这些删除既不会导致Ubx表达丢失，也不会导致功能丧失表型。因此，包括PRE在内的3kb区域是阻抑而非激活Ubx所必需的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号