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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Recognition of polyadenosine RNA by zinc finger proteins
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Recognition of polyadenosine RNA by zinc finger proteins

机译：锌指蛋白识别多腺苷RNA

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摘要

Messenger RNA transcripts are coated from cap to tail with a dynamic combination of RNA binding proteins that process, package, and ultimately regulate the fate of mature transcripts. One class of RNA binding proteins essential for multiple aspects of mRNA metabolism consists of the poly(A) binding proteins. Previous studies have concentrated on the canonical RNA recognition motif-containing poly(A) binding proteins as the sole family of poly(A)-specific RNA binding proteins. In this study, we present evidence for a previously uncharacterized poly(A) recognition motif consisting of tandem CCCH zinc fingers. We have probed the nucleic acid binding properties of a yeast protein, Nab2, that contains this zinc finger motif. Results of this study reveal that the seven tandem CCCH zinc fingers of Nab2 specifically bind to polyadenosine RNA with high affinity. Furthermore, we demonstrate that a human protein, ZC3H14, which contains CCCH zinc fingers homologous to those found in Nab2, also specifically binds polyadenosine RNA. Thus, we propose that these proteins are members of an evolutionarily conserved family of poly(A) RNA binding proteins that recognize poly(A) RNA through a fundamentally different mechanism than previously characterized RNA recognition motif-containing poly(A) binding proteins.
机译：Messenger RNA转录本由RNA结合蛋白的动态组合从头到尾覆盖，该结合蛋白加工，包装并最终调节成熟转录本的命运。对于mRNA代谢的多个方面必不可少的一类RNA结合蛋白由poly（A）结合蛋白组成。先前的研究集中于包含规范RNA识别基序的poly（A）结合蛋白，这是poly（A）特异性RNA结合蛋白的唯一家族。在这项研究中，我们为先前未鉴定的由串联CCCH锌指组成的poly（A）识别基序提供证据。我们已经探查了含有这种锌指基序的酵母蛋白Nab2的核酸结合特性。这项研究的结果表明，Nab2的七个串联CCCH锌指以高亲和力与聚腺苷RNA特异性结合。此外，我们证明了人类蛋白ZC3H14含有与Nab2中发现的CCCH锌指同源的CCCH锌指，它也能特异性结合聚腺苷RNA。因此，我们提出这些蛋白质是聚（A）RNA结合蛋白进化保守家族的成员，该聚（A）RNA结合蛋白通过与以前表征的含RNA识别基序的聚（A）结合蛋白根本不同的机制识别聚（A）RNA。

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