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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Structural basis for reversible photoswitching in Dronpa
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Structural basis for reversible photoswitching in Dronpa

机译：Dronpa中可逆光开关的结构基础

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摘要

Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state by irradiation with light. To elucidate the molecular basis of the switching mechanism, we generated reversibly switchable Dronpa protein crystals. Using these crystals we determined the elusive dark-state structure of Dronpa at 1.95-A resolution. We found that the photoswitching results in a cis-trans isomerization of the chromophore accompanied by complex structural rearrangements of four nearby amino acid residues. Because of this cascade of intramolecular events, the chromophore is exposed to distinct electrostatic surface potentials, which are likely to influence the protonation equilibria at the chromophore. We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore.
机译：Dronpa是一种新型的GFP样荧光蛋白，具有出色的光控开关特性。通过用光照射，可以在荧光导通状态和非荧光截止状态之间可逆地切换。为了阐明转换机制的分子基础，我们生成了可逆转换的Dronpa蛋白晶体。使用这些晶体，我们以1.95-A的分辨率确定了Dronpa难以捉摸的暗态结构。我们发现光开关导致发色团的顺反异构化，伴随着四个附近氨基酸残基的复杂结构重排。由于分子内事件的这种级联，发色团暴露于独特的静电表面电势，这很可能影响发色团处的质子平衡。我们建议光诱导开关机制的综合模型，将级联的结构重排与生色团的不同质子化状态联系起来。

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