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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >β-Catenin regulates excitatory postsynaptic strength at hippocampal synapses
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β-Catenin regulates excitatory postsynaptic strength at hippocampal synapses

机译：β-连环蛋白调节海马突触的兴奋性突触后强度

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摘要

The precise contribution of the cadherin-β-catenin synapse adhesion complex in the functional and structural changes associated with the pre- and postsynaptic terminals remains unclear. Here we report a requirement for endogenous β-catenin in regulating synaptic strength and dendritic spine morphology in cultured hippocampal pyramidal neurons. Ablating β-catenin after the initiation of synaptogenesis in the postsynaptic neuron reduces the amplitude of spontaneous excitatory synaptic responses without a concurrent change in their frequency and synapse density. The normal glutamatergic synaptic response is maintained by postsynaptic β-catenin in a cadherin-dependent manner and requires the C-terminal PDZ-binding motif of β-catenin but not the link to the actin cytoskeleton. In addition, ablating β-catenin in postsynaptic neurons accompanies a block of bidirectional quantal scaling of glutamatergic responses induced by chronic activity manipulation. In older cultures at a time when neurons have abundant dendritic spines, neurons ablated for β-catenin show thin, elongated spines and reduced proportion of mushroom spines without a change in spine density. Collectively, these findings suggest that the cad-herin-β-catenin complex is an integral component of synaptic strength regulation and plays a basic role in coupling synapse function and spine morphology.
机译：cadherin-β-catenin突触粘附复合物在与突触前和突触后末梢有关的功能和结构变化中的精确贡献尚不清楚。在这里，我们报告内源性β-连环蛋白在调节海马锥体神经元的突触强度和树突棘形态方面的需求。在突触后神经元突触发生后消融β-catenin可以降低自发性兴奋性突触反应的幅度，而不会同时改变其频率和突触密度。正常的谷氨酸能突触反应由突触后β-连环蛋白以钙粘着蛋白依赖性方式维持，并且需要β-连环蛋白的C末端PDZ结合基序，但不需要与肌动蛋白细胞骨架的联系。另外，消融突触后神经元中的β-catenin伴随着慢性活动操纵诱导的谷氨酸能反应的双向定量缩放。在较老的文化中，神经元具有丰富的树突棘，消融了β-catenin的神经元显示出细而细长的棘刺和蘑菇棘突的比例减少，而脊柱密度没有变化。总的来说，这些发现表明，cad-herin-β-catenin复合物是突触强度调节的组成部分，并且在偶联突触功能和脊柱形态中起基本作用。

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