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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A cyclic nucleotide-gated channel is essential for polarized tip growth of pollen
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A cyclic nucleotide-gated channel is essential for polarized tip growth of pollen

机译：环状核苷酸门控通道对于花粉的极化尖端生长至关重要

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Ion signals are critical to regulating polarized growth in many cell types, including pollen in plants and neurons in animals. Genetic evidence presented here indicates that pollen tube growth requires cyclic nucleotide-gated channel (CNGC) 18. CNGCs are nonspecific cation channels found in plants and animals and have well established functions in excitatory signal transduction events in animals. In Arabidopsis, male sterility was observed for two cngc18 null mutations. CNGC18 is expressed primarily in pollen, as indicated from a promoter.:GUS [β-glucuronidase) reporter analysis and expression profiling. The underlying cause of sterility was identified as a defect in pollen tube growth, resulting in tubes that were kinky, short, often thin, and unable to grow into the transmitting tract. Expression of a GFP-tagged CNGC18 in mutant pollen provided complementation and evidence for asymmetric localization of CNGC18 to the plasma membrane at the growing tip, starting at the time of pollen grain germination. Heterologous expression of CNGC18 in Escherichia coli resulted in a time- and concentration-dependent accumulation of more Ca~(2+). Thus, CNGC18 provides a mechanism to directly transduce a cyclic nu-cleotide (cNMP) signal into an ion flux that can produce a localized signal capable of regulating the pollen tip-growth machinery. These results identify a CNGC that is essential to an organism's life cycle and raise the possibility that CNGCs have a widespread role in regulating cell-growth dynamics in both plant and animals.
机译：离子信号对于调节许多细胞类型的极化生长至关重要，包括植物中的花粉和动物中的神经元。此处提供的遗传证据表明，花粉管的生长需要环状核苷酸门控通道（CNGC）18。CNGC是在动植物中发现的非特异性阳离子通道，在动物的兴奋性信号转导事件中具有公认的功能。在拟南芥中，观察到两个cngc18 null突变的雄性不育。 CNGC18主要在花粉中表达，如启动子所示：GUS [β-葡萄糖醛酸苷酶]报告基因分析和表达谱。不育的根本原因被确定为花粉管生长的缺陷，导致花管变短，变短，经常变薄并且无法长入传播管道。从花粉粒萌发时开始，GFP标记的CNGC18在突变的花粉中的表达提供了补充，并证明了CNGC18在生长尖端向质膜的不对称定位。 CNGC18在大肠杆菌中的异源表达导致更多Ca〜（2+）的时间和浓度依赖性积累。因此，CNGC18提供了一种机制，可直接将环状核苷酸（cNMP）信号转换为离子通量，该离子通量可产生能够调节花粉尖端生长机制的局部信号。这些结果确定了CNGC对生物的生命周期至关重要，并提高了CNGC在调节动植物中细胞生长动力学中广泛发挥作用的可能性。

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